Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 67717..68360 Replicon plasmid pCD306
Accession NZ_CP013832
Organism Escherichia coli strain CD306

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag AVR67_RS25830 Protein ID WP_001034044.1
Coordinates 67717..68133 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag AVR67_RS25835 Protein ID WP_001261286.1
Coordinates 68130..68360 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR67_RS25810 64121..64818 + 698 Protein_70 IS1 family transposase -
AVR67_RS25820 65072..66092 - 1021 Protein_71 DNA helicase UvrD -
AVR67_RS25825 66077..67642 - 1566 WP_001128474.1 AAA family ATPase -
AVR67_RS25830 67717..68133 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
AVR67_RS25835 68130..68360 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AVR67_RS26325 68741..69766 + 1026 Protein_75 pcar -
AVR67_RS25845 69773..70470 - 698 Protein_76 IS1 family transposase -
AVR67_RS25855 70719..71120 - 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
AVR67_RS29375 71053..71310 - 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
AVR67_RS25865 71403..72056 - 654 WP_000616807.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA5 / sitABCD / blaTEM-1B iucA / iucB / iucB / iucC / iucD / iutA 1..145221 145221
- flank IS/Tn - - 64315..64818 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T59416 WP_001034044.1 NZ_CP013832:c68133-67717 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T59416 NZ_CP013832:c68133-67717 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT59416 WP_001261286.1 NZ_CP013832:c68360-68130 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT59416 NZ_CP013832:c68360-68130 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References