Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 40237..40796 Replicon plasmid pCD306
Accession NZ_CP013832
Organism Escherichia coli strain CD306

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag AVR67_RS25680 Protein ID WP_000421272.1
Coordinates 40521..40796 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag AVR67_RS25675 Protein ID WP_001387467.1
Coordinates 40237..40521 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR67_RS25645 35700..36140 - 441 Protein_40 zinc-binding dehydrogenase -
AVR67_RS25655 36541..37431 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
AVR67_RS25660 37456..37836 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
AVR67_RS25665 37869..38834 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
AVR67_RS25670 38880..39632 - 753 WP_000562172.1 IclR family transcriptional regulator -
AVR67_RS25675 40237..40521 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
AVR67_RS25680 40521..40796 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AVR67_RS25685 40902..41195 + 294 WP_001105060.1 hypothetical protein -
AVR67_RS29360 41230..41358 - 129 Protein_48 IS481 family transposase -
AVR67_RS25690 41391..42560 - 1170 WP_001072355.1 hypothetical protein -
AVR67_RS25695 43053..43360 + 308 Protein_50 type II toxin-antitoxin system ParD family antitoxin -
AVR67_RS29365 43703..43885 + 183 WP_042344623.1 hypothetical protein -
AVR67_RS25710 44006..44746 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA5 / sitABCD / blaTEM-1B iucA / iucB / iucB / iucC / iucD / iutA 1..145221 145221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T59415 WP_000421272.1 NZ_CP013832:40521-40796 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T59415 NZ_CP013832:40521-40796 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT59415 WP_001387467.1 NZ_CP013832:40237-40521 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT59415 NZ_CP013832:40237-40521 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References