Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2311923..2312754 Replicon chromosome
Accession NZ_CP013831
Organism Escherichia coli strain CD306

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag AVR67_RS12015 Protein ID WP_000854815.1
Coordinates 2312380..2312754 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag AVR67_RS12010 Protein ID WP_001280918.1
Coordinates 2311923..2312291 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR67_RS11965 2307012..2307758 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
AVR67_RS11970 2307841..2308191 + 351 Protein_2301 hypothetical protein -
AVR67_RS11975 2308207..2308617 + 411 WP_000846703.1 hypothetical protein -
AVR67_RS11980 2308838..2309656 + 819 WP_001542275.1 DUF945 domain-containing protein -
AVR67_RS11985 2309656..2309901 + 246 WP_001164966.1 hypothetical protein -
AVR67_RS11990 2309995..2310468 + 474 WP_001542276.1 antirestriction protein -
AVR67_RS11995 2310484..2310960 + 477 WP_001186200.1 RadC family protein -
AVR67_RS12000 2311023..2311244 + 222 WP_000692345.1 DUF987 domain-containing protein -
AVR67_RS12005 2311263..2311907 + 645 WP_000086752.1 hypothetical protein -
AVR67_RS12010 2311923..2312291 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AVR67_RS12015 2312380..2312754 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
AVR67_RS12020 2312751..2312945 + 195 WP_000988600.1 hypothetical protein -
AVR67_RS29085 2312958..2313071 + 114 WP_001161660.1 DUF957 domain-containing protein -
AVR67_RS12030 2313360..2313506 - 147 Protein_2313 transposase domain-containing protein -
AVR67_RS12035 2313560..2313742 + 183 WP_001296208.1 hypothetical protein -
AVR67_RS12040 2313843..2314172 - 330 WP_000450409.1 DUF496 family protein -
AVR67_RS12045 2314344..2315402 - 1059 WP_001200889.1 FUSC family protein -
AVR67_RS12050 2315600..2316073 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
AVR67_RS12055 2316192..2317358 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T59402 WP_000854815.1 NZ_CP013831:2312380-2312754 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T59402 NZ_CP013831:2312380-2312754 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT59402 WP_001280918.1 NZ_CP013831:2311923-2312291 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT59402 NZ_CP013831:2311923-2312291 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References