Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 6841..7447 Replicon plasmid p2PS10
Accession NZ_CP013818
Organism Piscirickettsia salmonis strain AY3800B

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B8UI87
Locus tag AVM72_RS16085 Protein ID WP_027242955.1
Coordinates 6841..7101 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7U4WZG4
Locus tag AVM72_RS16090 Protein ID WP_016211078.1
Coordinates 7094..7447 (+) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVM72_RS16055 2101..3084 + 984 WP_016211499.1 ParB/RepB/Spo0J family partition protein -
AVM72_RS21030 3590..3865 + 276 WP_171824216.1 hypothetical protein -
AVM72_RS16065 4070..4513 + 444 WP_178099494.1 transposase -
AVM72_RS19065 4510..5484 - 975 WP_036771330.1 IS30 family transposase -
AVM72_RS21035 5598..5954 + 357 WP_075278739.1 transposase -
AVM72_RS16080 6020..6610 - 591 WP_016212329.1 site-specific integrase -
AVM72_RS16085 6841..7101 + 261 WP_027242955.1 type II toxin-antitoxin system HicA family toxin Toxin
AVM72_RS16090 7094..7447 + 354 WP_016211078.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AVM72_RS16095 7624..8598 + 975 WP_069971681.1 IS30 family transposase -
AVM72_RS16100 8707..8934 + 228 Protein_11 hypothetical protein -
AVM72_RS16105 9131..9496 + 366 WP_027242954.1 hypothetical protein -
AVM72_RS16110 9641..9895 + 255 WP_027242953.1 type II toxin-antitoxin system HicA family toxin -
AVM72_RS16115 9888..10235 + 348 WP_027242952.1 type II toxin-antitoxin system HicB family antitoxin -
AVM72_RS19070 10333..11307 + 975 WP_036771330.1 IS30 family transposase -
AVM72_RS21040 11539..11685 - 147 WP_169834793.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33556 33556


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-82)

Antitoxin

Uncharacterised protein family HicB

(53-103)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9695.31 Da        Isoelectric Point: 10.9468

>T59374 WP_027242955.1 NZ_CP013818:6841-7101 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Download         Length: 261 bp

>T59374 NZ_CP013818:6841-7101 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13492.39 Da        Isoelectric Point: 5.0200

>AT59374 WP_016211078.1 NZ_CP013818:7094-7447 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Download         Length: 354 bp

>AT59374 NZ_CP013818:7094-7447 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B8UI87

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4WZG4

References