Detailed information of TA system TA059360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2500169..2500932
• Replicon: chromosome
• Accession: NZ_CP013806
• Organism: Piscirickettsia salmonis strain PM31429B

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A1L6TH26
• Locus tag: AVM74_RS12870
• Protein ID: WP_017376502.1
• Coordinates: 2500423..2500932 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: AVM74_RS12865
• Protein ID: WP_017376501.1
• Coordinates: 2500169..2500435 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVM74_RS12840	2495808..2496395	+	588	Protein_2494	IS982-like element ISPsa1 family transposase	-
AVM74_RS12845	2496519..2497922	+	1404	WP_048876031.1	IS4 family transposase	-
AVM74_RS12850	2497936..2498232	+	297	Protein_2496	transposase	-
AVM74_RS12855	2498301..2498849	+	549	WP_196480236.1	transposase	-
AVM74_RS12860	2499029..2500003	+	975	WP_048875903.1	IS30 family transposase	-
AVM74_RS12865	2500169..2500435	+	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
AVM74_RS12870	2500423..2500932	+	510	WP_017376502.1	GNAT family N-acetyltransferase	Toxin
AVM74_RS12875	2501053..2501928	-	876	WP_048875904.1	IS982 family transposase	-
AVM74_RS20305	2502122..2502427	-	306	WP_146619445.1	transposase	-
AVM74_RS20310	2502431..2503093	-	663	WP_174872532.1	transposase	-
AVM74_RS18545	2503018..2503257	-	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
AVM74_RS12895	2503588..2504118	-	531	WP_017375999.1	transcriptional repressor	-
AVM74_RS12900	2504118..2504645	-	528	WP_026063500.1	inorganic diphosphatase	-
AVM74_RS12905	2504805..2505920	-	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2495808..2501928	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (70-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18875.95 Da
• Isoelectric Point: 8.6859

>T59360 WP_017376502.1 NZ_CP013806:2500423-2500932 [Piscirickettsia salmonis]
MGEITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPE
TIPVIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKI
RQLLKNETK

Nucleotide

• Length: 510 bp

>T59360 NZ_CP013806:2500423-2500932 [Piscirickettsia salmonis]
TTGGGAGAAATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAA
TGAGTGGTTACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAG
TGATTGGCTATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAG
ACAATTCCGGTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAA
AGATGCTTTAATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAG
CAAAACAGTTTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATT
AGGCAACTTCTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT59360 WP_017376501.1 NZ_CP013806:2500169-2500435 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT59360 NZ_CP013806:2500169-2500435 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Toxin

• AlphaFold DB: A0A1L6TH26

Antitoxin

• AlphaFold DB: A0A1L6TGZ1