Detailed information of TA system TA059348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 225831..226594
• Replicon: chromosome
• Accession: NZ_CP013796
• Organism: Piscirickettsia salmonis strain AY6532B

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A1L6TH26
• Locus tag: AVI56_RS01205
• Protein ID: WP_017376502.1
• Coordinates: 225831..226340 (-)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: AVI56_RS01210
• Protein ID: WP_017376501.1
• Coordinates: 226328..226594 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI56_RS01170	220843..221958	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
AVI56_RS01175	222118..222645	+	528	WP_026063500.1	inorganic diphosphatase	-
AVI56_RS01180	222645..223175	+	531	WP_017375999.1	transcriptional repressor	-
AVI56_RS18200	223506..223745	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
AVI56_RS19955	223670..224332	+	663	WP_174872532.1	transposase	-
AVI56_RS19960	224336..224641	+	306	WP_146619445.1	transposase	-
AVI56_RS01200	224835..225710	+	876	WP_048875904.1	IS982 family transposase	-
AVI56_RS01205	225831..226340	-	510	WP_017376502.1	GNAT family N-acetyltransferase	Toxin
AVI56_RS01210	226328..226594	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
AVI56_RS01215	226760..227734	-	975	WP_048875903.1	IS30 family transposase	-
AVI56_RS01220	227914..228534	-	621	WP_075275269.1	transposase	-
AVI56_RS01225	228531..228827	-	297	Protein_239	transposase	-
AVI56_RS01230	228841..230244	-	1404	WP_048876031.1	IS4 family transposase	-
AVI56_RS01235	230368..230955	-	588	Protein_241	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	224835..230955	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (70-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18875.95 Da
• Isoelectric Point: 8.6859

>T59348 WP_017376502.1 NZ_CP013796:c226340-225831 [Piscirickettsia salmonis]
MGEITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPE
TIPVIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKI
RQLLKNETK

Nucleotide

• Length: 510 bp

>T59348 NZ_CP013796:c226340-225831 [Piscirickettsia salmonis]
TTGGGAGAAATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAA
TGAGTGGTTACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAG
TGATTGGCTATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAG
ACAATTCCGGTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAA
AGATGCTTTAATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAG
CAAAACAGTTTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATT
AGGCAACTTCTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT59348 WP_017376501.1 NZ_CP013796:c226594-226328 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT59348 NZ_CP013796:c226594-226328 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Toxin

• AlphaFold DB: A0A1L6TH26

Antitoxin

• AlphaFold DB: A0A1L6TGZ1