Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 6842..7448 Replicon plasmid p3PS8
Accession NZ_CP013794
Organism Piscirickettsia salmonis strain AY6297B

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B8UI87
Locus tag AVI55_RS17635 Protein ID WP_027242955.1
Coordinates 6842..7102 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7U4WZG4
Locus tag AVI55_RS17640 Protein ID WP_016211078.1
Coordinates 7095..7448 (+) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVI55_RS17605 2102..3085 + 984 WP_016211499.1 ParB/RepB/Spo0J family partition protein -
AVI55_RS19995 3591..3866 + 276 WP_171824216.1 hypothetical protein -
AVI55_RS17615 4071..4514 + 444 WP_178099494.1 transposase -
AVI55_RS17620 4511..5485 - 975 WP_036771330.1 IS30 family transposase -
AVI55_RS20000 5599..5955 + 357 WP_075278739.1 transposase -
AVI55_RS17630 6021..6611 - 591 WP_016212329.1 site-specific integrase -
AVI55_RS17635 6842..7102 + 261 WP_027242955.1 type II toxin-antitoxin system HicA family toxin Toxin
AVI55_RS17640 7095..7448 + 354 WP_016211078.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AVI55_RS17645 7625..8599 + 975 WP_069971681.1 IS30 family transposase -
AVI55_RS17650 8708..8935 + 228 Protein_11 hypothetical protein -
AVI55_RS17655 9132..9497 + 366 WP_027242954.1 hypothetical protein -
AVI55_RS17660 9642..9896 + 255 WP_027242953.1 type II toxin-antitoxin system HicA family toxin -
AVI55_RS17665 9889..10236 + 348 WP_027242952.1 type II toxin-antitoxin system HicB family antitoxin -
AVI55_RS17670 10334..11308 + 975 WP_036771330.1 IS30 family transposase -
AVI55_RS20005 11540..11686 - 147 WP_169834793.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33557 33557


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-82)

Antitoxin

Uncharacterised protein family HicB

(53-103)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9695.31 Da        Isoelectric Point: 10.9468

>T59347 WP_027242955.1 NZ_CP013794:6842-7102 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Download         Length: 261 bp

>T59347 NZ_CP013794:6842-7102 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13492.39 Da        Isoelectric Point: 5.0200

>AT59347 WP_016211078.1 NZ_CP013794:7095-7448 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Download         Length: 354 bp

>AT59347 NZ_CP013794:7095-7448 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B8UI87

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4WZG4

References