Detailed information of TA system TA059341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 9907..10513
• Replicon: plasmid p4PS7
• Accession: NZ_CP013790
• Organism: Piscirickettsia salmonis strain PM58386B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVI54_RS18085
• Protein ID: WP_027242955.1
• Coordinates: 10253..10513 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: AVI54_RS18080
• Protein ID: WP_016211078.1
• Coordinates: 9907..10260 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI54_RS20570	5675..5815	+	141	WP_162039123.1	hypothetical protein	-
AVI54_RS18050	6047..7021	-	975	WP_036771330.1	IS30 family transposase	-
AVI54_RS18055	7119..7466	-	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
AVI54_RS18060	7459..7713	-	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
AVI54_RS18065	7858..8223	-	366	WP_027242954.1	hypothetical protein	-
AVI54_RS18070	8420..8647	-	228	Protein_14	hypothetical protein	-
AVI54_RS18075	8756..9730	-	975	WP_069971681.1	IS30 family transposase	-
AVI54_RS18080	9907..10260	-	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVI54_RS18085	10253..10513	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVI54_RS18090	10744..11334	+	591	WP_016212329.1	site-specific integrase	-
AVI54_RS18095	11400..11756	-	357	WP_075278739.1	transposase	-
AVI54_RS18100	11870..12844	+	975	WP_036771330.1	IS30 family transposase	-
AVI54_RS18105	12841..13284	-	444	WP_178099494.1	transposase	-
AVI54_RS20575	13489..13764	-	276	WP_171824216.1	hypothetical protein	-
AVI54_RS18115	14270..15253	-	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33555	33555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59341 WP_027242955.1 NZ_CP013790:c10513-10253 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59341 NZ_CP013790:c10513-10253 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT59341 WP_016211078.1 NZ_CP013790:c10260-9907 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT59341 NZ_CP013790:c10260-9907 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4