Detailed information of TA system TA059325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 24892..25535
• Replicon: plasmid p3PS4
• Accession: NZ_CP013776
• Organism: Piscirickettsia salmonis strain PM37984A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: AVI51_RS16935
• Protein ID: WP_016210974.1
• Coordinates: 24892..25299 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: AVI51_RS16940
• Protein ID: WP_016210972.1
• Coordinates: 25308..25535 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI51_RS16905	21218..21697	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
AVI51_RS16910	21785..22168	-	384	WP_032126134.1	HNH endonuclease	-
AVI51_RS16915	22487..22852	-	366	WP_016212235.1	hypothetical protein	-
AVI51_RS18430	22899..23078	+	180	WP_155046773.1	phosphatase	-
AVI51_RS18435	23223..23405	+	183	WP_155047026.1	hypothetical protein	-
AVI51_RS16925	23623..23916	-	294	WP_016210977.1	zinc ribbon domain-containing protein	-
AVI51_RS16930	24095..24775	-	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
AVI51_RS16935	24892..25299	-	408	WP_016210974.1	DUF4411 family protein	Toxin
AVI51_RS16940	25308..25535	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AVI51_RS16945	25672..26646	-	975	WP_054300271.1	IS30 family transposase	-
AVI51_RS16950	26872..27963	-	1092	WP_075274766.1	hypothetical protein	-
AVI51_RS16955	28494..28958	-	465	WP_016212274.1	type II toxin-antitoxin system HicB family antitoxin	-
AVI51_RS16960	28969..29163	-	195	WP_032126154.1	type II toxin-antitoxin system HicA family toxin	-
AVI51_RS16965	29379..29969	+	591	WP_032126152.1	site-specific integrase	-
AVI51_RS16970	30033..30374	+	342	WP_081007075.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36245	36245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T59325 WP_016210974.1 NZ_CP013776:c25299-24892 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T59325 NZ_CP013776:c25299-24892 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT59325 WP_016210972.1 NZ_CP013776:c25535-25308 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT59325 NZ_CP013776:c25535-25308 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5