Detailed information of TA system TA059324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 20576..21182
• Replicon: plasmid p2PS4
• Accession: NZ_CP013775
• Organism: Piscirickettsia salmonis strain PM37984A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVI51_RS16665
• Protein ID: WP_027242955.1
• Coordinates: 20576..20836 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AVI51_RS16670
• Protein ID: WP_054300685.1
• Coordinates: 20829..21182 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI51_RS16635	15700..15924	-	225	Protein_22	hypothetical protein	-
AVI51_RS16640	16563..17555	+	993	WP_054300689.1	ParA family protein	-
AVI51_RS16645	17572..18939	+	1368	WP_054300688.1	hypothetical protein	-
AVI51_RS16650	19303..19557	+	255	WP_054300687.1	DUF4160 domain-containing protein	-
AVI51_RS16655	19600..19833	+	234	WP_036781101.1	hypothetical protein	-
AVI51_RS16660	19880..20314	+	435	WP_054300686.1	hypothetical protein	-
AVI51_RS16665	20576..20836	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVI51_RS16670	20829..21182	+	354	WP_054300685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVI51_RS16675	21286..22086	+	801	WP_054300684.1	methyltransferase domain-containing protein	-
AVI51_RS16680	22313..23395	+	1083	WP_054300162.1	IS481 family transposase	-
AVI51_RS16685	23425..24078	-	654	WP_155764092.1	hypothetical protein	-
AVI51_RS16690	24253..25224	-	972	WP_033923677.1	IS91 family transposase	-
AVI51_RS17705	25149..25388	-	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36081	36081
	-	flank	IS/Tn	-	-	22313..23395	1082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59324 WP_027242955.1 NZ_CP013775:20576-20836 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59324 NZ_CP013775:20576-20836 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13462.36 Da
• Isoelectric Point: 5.0200

>AT59324 WP_054300685.1 NZ_CP013775:20829-21182 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGGDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT59324 NZ_CP013775:20829-21182 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTGGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTAAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87