Detailed information of TA system TA059321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 7285..7891
• Replicon: plasmid p3PS3
• Accession: NZ_CP013771
• Organism: Piscirickettsia salmonis strain PM23019A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVI50_RS17025
• Protein ID: WP_027242955.1
• Coordinates: 7285..7545 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AVI50_RS17030
• Protein ID: WP_054300685.1
• Coordinates: 7538..7891 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI50_RS16985	3278..3811	+	534	WP_075286012.1	AAA family ATPase	-
AVI50_RS16990	3808..4185	+	378	WP_075286014.1	ParA family protein	-
AVI50_RS17000	4778..4969	+	192	WP_075286018.1	hypothetical protein	-
AVI50_RS17005	5347..5649	+	303	WP_155764091.1	hypothetical protein	-
AVI50_RS17010	6012..6266	+	255	WP_054300687.1	DUF4160 domain-containing protein	-
AVI50_RS17015	6309..6542	+	234	WP_036781101.1	hypothetical protein	-
AVI50_RS17020	6589..7023	+	435	WP_054300686.1	hypothetical protein	-
AVI50_RS17025	7285..7545	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVI50_RS17030	7538..7891	+	354	WP_054300685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVI50_RS17035	7995..8795	+	801	WP_054300684.1	methyltransferase domain-containing protein	-
AVI50_RS17040	9022..10104	+	1083	WP_054300162.1	IS481 family transposase	-
AVI50_RS17045	10134..10787	-	654	WP_155764092.1	hypothetical protein	-
AVI50_RS17050	10962..11933	-	972	WP_033923677.1	IS91 family transposase	-
AVI50_RS17995	11858..12097	-	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36063	36063
	-	flank	IS/Tn	-	-	9022..10104	1082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59321 WP_027242955.1 NZ_CP013771:7285-7545 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59321 NZ_CP013771:7285-7545 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13462.36 Da
• Isoelectric Point: 5.0200

>AT59321 WP_054300685.1 NZ_CP013771:7538-7891 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGGDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT59321 NZ_CP013771:7538-7891 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTGGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTAAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87