Detailed information of TA system TA059320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 30238..30881
• Replicon: plasmid p2PS3
• Accession: NZ_CP013770
• Organism: Piscirickettsia salmonis strain PM23019A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: AVI50_RS16915
• Protein ID: WP_016210974.1
• Coordinates: 30238..30645 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: AVI50_RS16920
• Protein ID: WP_016210972.1
• Coordinates: 30654..30881 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI50_RS16885	26567..27046	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
AVI50_RS18760	27134..27516	-	383	Protein_36	HNH endonuclease	-
AVI50_RS16895	27834..28151	-	318	WP_129556723.1	hypothetical protein	-
AVI50_RS18765	28569..28751	+	183	WP_155047026.1	hypothetical protein	-
AVI50_RS16905	28969..29262	-	294	WP_016210977.1	zinc ribbon domain-containing protein	-
AVI50_RS16910	29441..30121	-	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
AVI50_RS16915	30238..30645	-	408	WP_016210974.1	DUF4411 family protein	Toxin
AVI50_RS16920	30654..30881	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AVI50_RS16925	31018..31992	-	975	WP_054300271.1	IS30 family transposase	-
AVI50_RS16930	32218..32412	-	195	WP_075286007.1	hypothetical protein	-
AVI50_RS16935	32451..33308	-	858	WP_075286009.1	hypothetical protein	-
AVI50_RS16940	33839..34303	-	465	WP_016212274.1	type II toxin-antitoxin system HicB family antitoxin	-
AVI50_RS16945	34314..34508	-	195	WP_032126154.1	type II toxin-antitoxin system HicA family toxin	-
AVI50_RS16950	34724..35314	+	591	WP_032126152.1	site-specific integrase	-
AVI50_RS16955	35378..35719	+	342	WP_081007075.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36218	36218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T59320 WP_016210974.1 NZ_CP013770:c30645-30238 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T59320 NZ_CP013770:c30645-30238 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT59320 WP_016210972.1 NZ_CP013770:c30881-30654 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT59320 NZ_CP013770:c30881-30654 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5