Detailed information of TA system TA059317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 26656..27262
• Replicon: plasmid p3PS2
• Accession: NZ_CP013765
• Organism: Piscirickettsia salmonis strain PM21567A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVI49_RS16980
• Protein ID: WP_027242955.1
• Coordinates: 26656..26916 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AVI49_RS16985
• Protein ID: WP_036780061.1
• Coordinates: 26909..27262 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI49_RS16965	22886..23911	+	1026	WP_032126388.1	helix-turn-helix domain-containing protein	-
AVI49_RS16970	24024..25256	+	1233	WP_032126623.1	MFS transporter	-
AVI49_RS16975	25433..26407	-	975	WP_054300271.1	IS30 family transposase	-
AVI49_RS16980	26656..26916	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVI49_RS16985	26909..27262	+	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVI49_RS18815	27350..27577	+	228	WP_155047027.1	hypothetical protein	-
AVI49_RS16990	27725..28696	-	972	WP_033923677.1	IS91 family transposase	-
AVI49_RS18005	28621..28920	-	300	WP_075274768.1	transposase zinc-binding domain-containing protein	-
AVI49_RS17000	29175..29633	+	459	WP_054300586.1	hypothetical protein	-
AVI49_RS17005	29808..30779	-	972	WP_033923677.1	IS91 family transposase	-
AVI49_RS18010	30704..31003	-	300	WP_075274768.1	transposase zinc-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39331	39331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59317 WP_027242955.1 NZ_CP013765:26656-26916 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59317 NZ_CP013765:26656-26916 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT59317 WP_036780061.1 NZ_CP013765:26909-27262 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT59317 NZ_CP013765:26909-27262 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTGAAGCATGATCAAATCGTTAAACGGTAA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87