Detailed information of TA system TA059316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 6257..6900
• Replicon: plasmid p3PS2
• Accession: NZ_CP013765
• Organism: Piscirickettsia salmonis strain PM21567A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: AVI49_RS16865
• Protein ID: WP_016210974.1
• Coordinates: 6493..6900 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: AVI49_RS16860
• Protein ID: WP_016210972.1
• Coordinates: 6257..6484 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI49_RS16830	1418..1759	-	342	WP_081007075.1	MBL fold metallo-hydrolase	-
AVI49_RS16835	1823..2413	-	591	WP_032126152.1	site-specific integrase	-
AVI49_RS16840	2629..2823	+	195	WP_032126154.1	type II toxin-antitoxin system HicA family toxin	-
AVI49_RS16845	2834..3298	+	465	WP_016212274.1	type II toxin-antitoxin system HicB family antitoxin	-
AVI49_RS16850	3829..4920	+	1092	WP_054300578.1	hypothetical protein	-
AVI49_RS16855	5146..6120	+	975	WP_054300271.1	IS30 family transposase	-
AVI49_RS16860	6257..6484	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AVI49_RS16865	6493..6900	+	408	WP_016210974.1	DUF4411 family protein	Toxin
AVI49_RS16870	7017..7697	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
AVI49_RS16875	7876..8169	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
AVI49_RS19130	8169..8447	+	279	WP_032126476.1	hypothetical protein	-
AVI49_RS18810	8714..8893	-	180	WP_155046773.1	phosphatase	-
AVI49_RS16885	8988..9305	+	318	WP_129556723.1	hypothetical protein	-
AVI49_RS16890	9615..9998	+	384	WP_032126134.1	HNH endonuclease	-
AVI49_RS16895	10086..10565	+	480	WP_016212234.1	P27 family phage terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39331	39331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T59316 WP_016210974.1 NZ_CP013765:6493-6900 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T59316 NZ_CP013765:6493-6900 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT59316 WP_016210972.1 NZ_CP013765:6257-6484 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT59316 NZ_CP013765:6257-6484 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5