Detailed information of TA system TA059315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 23129..23699
• Replicon: plasmid p2PS2
• Accession: NZ_CP013764
• Organism: Piscirickettsia salmonis strain PM21567A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AVI49_RS16480
• Protein ID: WP_054300674.1
• Coordinates: 23373..23699 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVI49_RS16475
• Protein ID: WP_054300649.1
• Coordinates: 23129..23371 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI49_RS16445	20190..20747	+	558	WP_054300675.1	recombinase family protein	-
AVI49_RS16450	20809..21069	+	261	WP_054300654.1	hypothetical protein	-
AVI49_RS16455	21119..21427	+	309	WP_081007087.1	helix-turn-helix transcriptional regulator	-
AVI49_RS16460	21526..21933	+	408	WP_054300652.1	hypothetical protein	-
AVI49_RS16465	21940..22374	+	435	WP_054300651.1	hypothetical protein	-
AVI49_RS16470	22395..22715	+	321	WP_054300650.1	hypothetical protein	-
AVI49_RS16475	23129..23371	+	243	WP_054300649.1	hypothetical protein	Antitoxin
AVI49_RS16480	23373..23699	+	327	WP_054300674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI49_RS16485	23816..25051	-	1236	WP_054300648.1	hypothetical protein	-
AVI49_RS16490	25077..26777	-	1701	WP_054300647.1	DUF4338 domain-containing protein	-
AVI49_RS16495	26890..27189	-	300	WP_075273685.1	HU family DNA-binding protein	-
AVI49_RS19115	27375..27599	+	225	WP_196214208.1	hypothetical protein	-
AVI49_RS19120	27593..27769	+	177	WP_172673426.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82125	82125
	-	flank	IS/Tn	-	-	20169..20747	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12091.96 Da
• Isoelectric Point: 9.9714

>T59315 WP_054300674.1 NZ_CP013764:23373-23699 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMFRG

Nucleotide

• Length: 327 bp

>T59315 NZ_CP013764:23373-23699 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTAACTTCCAAGCCTTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGCGCAGTGCTAGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGACTGGAATCATGCTGATAAGCTCGATAAAAAATCACTTGAAAGAGTTCAAGGTTTAATTGACGTGATGTTTAG
AGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.14 Da
• Isoelectric Point: 4.6318

>AT59315 WP_054300649.1 NZ_CP013764:23129-23371 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTKTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT59315 NZ_CP013764:23129-23371 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGCTTGGGTTTATTATTGCCATTGAGCATTGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACGCATACGAAGACCCTAGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA