Detailed information of TA system TA059312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 147737..148307
• Replicon: plasmid p4PS1
• Accession: NZ_CP013761
• Organism: Piscirickettsia salmonis strain AY6492A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AVI48_RS17465
• Protein ID: WP_036781349.1
• Coordinates: 147737..148063 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVI48_RS17470
• Protein ID: WP_016212365.1
• Coordinates: 148065..148307 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI48_RS17430	143413..143790	+	378	Protein_192	DDE-type integrase/transposase/recombinase	-
AVI48_RS17435	143891..144670	+	780	WP_081377351.1	transposase	-
AVI48_RS17445	144859..145191	+	333	WP_032126739.1	transposase	-
AVI48_RS17450	145356..145733	+	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
AVI48_RS17455	146040..146423	-	384	WP_016212152.1	hypothetical protein	-
AVI48_RS17460	146893..147621	-	729	WP_075273842.1	IS6 family transposase	-
AVI48_RS17465	147737..148063	-	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI48_RS17470	148065..148307	-	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
AVI48_RS17475	148512..148952	+	441	Protein_200	tyrosine-type recombinase/integrase	-
AVI48_RS17480	149020..149487	+	468	Protein_201	IS6-like element ISPsa2 family transposase	-
AVI48_RS18895	149661..149840	-	180	WP_155047017.1	hypothetical protein	-
AVI48_RS17485	149767..150036	+	270	Protein_203	DDE-type integrase/transposase/recombinase	-
AVI48_RS17490	150319..151953	+	1635	WP_075273844.1	protein kinase	-
AVI48_RS19215	152074..152805	+	732	WP_016212311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..158156	158156
	-	inside	IScluster/Tn	-	-	138405..153693	15288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T59312 WP_036781349.1 NZ_CP013761:c148063-147737 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T59312 NZ_CP013761:c148063-147737 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTGACTTCCAAGCCCTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGAGCAGTGTTGGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGGCTGGAATCATGCTGATAAGCTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGCTAATTGATGTGATGTTGAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT59312 WP_016212365.1 NZ_CP013761:c148307-148065 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT59312 NZ_CP013761:c148307-148065 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGGAATAGCTTGGGGTTGTTGCTACCCTTGAGCATCGCACGTGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACTCATACGCAGACCCTTGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA