Detailed information of TA system TA059310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 24529..25135
• Replicon: plasmid p2PS1
• Accession: NZ_CP013759
• Organism: Piscirickettsia salmonis strain AY6492A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVI48_RS16150
• Protein ID: WP_027242955.1
• Coordinates: 24875..25135 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AVI48_RS16145
• Protein ID: WP_036780061.1
• Coordinates: 24529..24882 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI48_RS18765	19870..20088	-	219	WP_155764037.1	hypothetical protein	-
AVI48_RS17970	20004..20630	-	627	WP_081377339.1	hypothetical protein	-
AVI48_RS17975	20848..21087	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
AVI48_RS16125	21012..21983	+	972	WP_033923677.1	IS91 family transposase	-
AVI48_RS16130	22158..22616	-	459	WP_054300586.1	hypothetical protein	-
AVI48_RS17980	22931..23170	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
AVI48_RS16140	23095..24066	+	972	WP_033923677.1	IS91 family transposase	-
AVI48_RS18770	24214..24441	-	228	WP_155047027.1	hypothetical protein	-
AVI48_RS16145	24529..24882	-	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVI48_RS16150	24875..25135	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVI48_RS16155	25384..26357	+	974	Protein_40	IS30 family transposase	-
AVI48_RS16160	26534..27766	-	1233	WP_032126623.1	MFS transporter	-
AVI48_RS16165	27879..28904	-	1026	WP_032126388.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39299	39299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59310 WP_027242955.1 NZ_CP013759:c25135-24875 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59310 NZ_CP013759:c25135-24875 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT59310 WP_036780061.1 NZ_CP013759:c24882-24529 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT59310 NZ_CP013759:c24882-24529 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTGAAGCATGATCAAATCGTTAAACGGTAA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87