Detailed information of TA system TA059309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 78606..79176
• Replicon: plasmid p1PS1
• Accession: NZ_CP013758
• Organism: Piscirickettsia salmonis strain AY6492A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AVI48_RS15940
• Protein ID: WP_054300674.1
• Coordinates: 78606..78932 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVI48_RS15945
• Protein ID: WP_054300649.1
• Coordinates: 78934..79176 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI48_RS19170	74536..74712	-	177	WP_172673426.1	transposase	-
AVI48_RS19175	74706..74930	-	225	WP_196214208.1	hypothetical protein	-
AVI48_RS15925	75116..75415	+	300	WP_075273685.1	HU family DNA-binding protein	-
AVI48_RS15930	75528..77228	+	1701	WP_054300647.1	DUF4338 domain-containing protein	-
AVI48_RS15935	77254..78489	+	1236	WP_054300648.1	hypothetical protein	-
AVI48_RS15940	78606..78932	-	327	WP_054300674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVI48_RS15945	78934..79176	-	243	WP_054300649.1	hypothetical protein	Antitoxin
AVI48_RS15950	79590..79910	-	321	WP_054300650.1	hypothetical protein	-
AVI48_RS15955	79931..80365	-	435	WP_054300651.1	hypothetical protein	-
AVI48_RS15960	80372..80779	-	408	WP_054300652.1	hypothetical protein	-
AVI48_RS15965	80878..81186	-	309	WP_081007087.1	helix-turn-helix transcriptional regulator	-
AVI48_RS15970	81236..81496	-	261	WP_054300654.1	hypothetical protein	-
AVI48_RS15975	81558..82115	-	558	WP_054300675.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82119	82119
	-	flank	IS/Tn	-	-	81558..82115	557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12091.96 Da
• Isoelectric Point: 9.9714

>T59309 WP_054300674.1 NZ_CP013758:c78932-78606 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMFRG

Nucleotide

• Length: 327 bp

>T59309 NZ_CP013758:c78932-78606 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTAACTTCCAAGCCTTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGCGCAGTGCTAGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGACTGGAATCATGCTGATAAGCTCGATAAAAAATCACTTGAAAGAGTTCAAGGTTTAATTGACGTGATGTTTAG
AGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.14 Da
• Isoelectric Point: 4.6318

>AT59309 WP_054300649.1 NZ_CP013758:c79176-78934 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTKTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT59309 NZ_CP013758:c79176-78934 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGCTTGGGTTTATTATTGCCATTGAGCATTGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACGCATACGAAGACCCTAGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA