Detailed information of TA system TA059284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2229777..2230403
• Replicon: chromosome
• Accession: NZ_CP013741
• Organism: Mycobacterium tuberculosis variant bovis strain BCG-1 (Russia)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BOVR_RS10395
• Protein ID: WP_003410075.1
• Coordinates: 2230005..2230403 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BOVR_RS10390
• Protein ID: WP_019283586.1
• Coordinates: 2229777..2230004 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOVR_RS10380	2227816..2228160	-	345	WP_003410065.1	ferredoxin family protein	-
BOVR_RS10385	2228349..2229518	-	1170	WP_003899126.1	ATP-binding protein	-
BOVR_RS10390	2229777..2230004	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BOVR_RS10395	2230005..2230403	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BOVR_RS10400	2230586..2231017	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BOVR_RS10405	2231118..2231552	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BOVR_RS20990	2231989..2232168	-	180	Protein_2035	hypothetical protein	-
BOVR_RS10410	2232229..2232873	+	645	WP_078396697.1	transposase	-
BOVR_RS10415	2232827..2233417	+	591	WP_003899131.1	IS110 family transposase	-
BOVR_RS10420	2233545..2234801	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T59284 WP_003410075.1 NZ_CP013741:2230005-2230403 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T59284 NZ_CP013741:2230005-2230403 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT59284 WP_019283586.1 NZ_CP013741:2229777-2230004 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT59284 NZ_CP013741:2229777-2230004 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG