Detailed information of TA system TA059278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2179094..2179797
• Replicon: chromosome
• Accession: NZ_CP013741
• Organism: Mycobacterium tuberculosis variant bovis strain BCG-1 (Russia)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BOVR_RS10080
• Protein ID: WP_003409778.1
• Coordinates: 2179094..2179423 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BOVR_RS20965
• Protein ID: WP_003409780.1
• Coordinates: 2179420..2179797 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOVR_RS10060	2175477..2176547	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BOVR_RS10065	2176544..2177059	+	516	WP_003409718.1	flavin reductase family protein	-
BOVR_RS10070	2177056..2178117	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BOVR_RS10075	2178114..2178884	+	771	WP_003409775.1	SDR family oxidoreductase	-
BOVR_RS10080	2179094..2179423	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOVR_RS20965	2179420..2179797	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BOVR_RS10090	2179794..2180384	-	591	WP_003910477.1	SEC-C domain-containing protein	-
BOVR_RS10095	2180439..2181803	+	1365	WP_011799233.1	HNH endonuclease	-
BOVR_RS10100	2181958..2182410	-	453	WP_010950636.1	lipoprotein	-
BOVR_RS10105	2182474..2182875	+	402	WP_003409869.1	hypothetical protein	-
BOVR_RS10110	2182868..2183050	-	183	WP_003409870.1	hypothetical protein	-
BOVR_RS10115	2183164..2183514	-	351	WP_003409871.1	hypothetical protein	-
BOVR_RS10120	2183525..2184427	-	903	WP_003409874.1	hypothetical protein	-
BOVR_RS10125	2184448..2184639	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T59278 WP_003409778.1 NZ_CP013741:c2179423-2179094 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T59278 NZ_CP013741:c2179423-2179094 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT59278 WP_003409780.1 NZ_CP013741:c2179797-2179420 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT59278 NZ_CP013741:c2179797-2179420 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8