Detailed information of TA system TA059243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3220030..3220723
• Replicon: chromosome
• Accession: NZ_CP013737
• Organism: Herbaspirillum rubrisubalbicans M1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A0U3LK25
• Locus tag: HRUBRI_RS14055
• Protein ID: WP_058895879.1
• Coordinates: 3220030..3220407 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0U3CND7
• Locus tag: HRUBRI_RS14060
• Protein ID: WP_017450286.1
• Coordinates: 3220388..3220723 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRUBRI_RS14030	3216188..3216856	+	669	WP_058895875.1	GntR family transcriptional regulator	-
HRUBRI_RS14035	3216882..3217781	-	900	WP_017450291.1	pseudouridine synthase	-
HRUBRI_RS14040	3217778..3219115	-	1338	WP_058895876.1	type II toxin-antitoxin system HipA family toxin	-
HRUBRI_RS14045	3219112..3219414	-	303	WP_058895877.1	helix-turn-helix domain-containing protein	-
HRUBRI_RS14050	3219635..3219823	-	189	WP_058895878.1	YjfB family protein	-
HRUBRI_RS14055	3220030..3220407	+	378	WP_058895879.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRUBRI_RS14060	3220388..3220723	+	336	WP_017450286.1	helix-turn-helix domain-containing protein	Antitoxin
HRUBRI_RS14065	3220800..3220988	-	189	WP_017450285.1	hypothetical protein	-
HRUBRI_RS14070	3221177..3222019	-	843	WP_058895880.1	anti-sigma factor	-
HRUBRI_RS14075	3222016..3222573	-	558	WP_058895881.1	RNA polymerase sigma factor	-
HRUBRI_RS14080	3222591..3222962	-	372	WP_017450282.1	hypothetical protein	-
HRUBRI_RS14085	3223157..3225586	-	2430	WP_058895882.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-96)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13979.15 Da
• Isoelectric Point: 9.8096

>T59243 WP_058895879.1 NZ_CP013737:3220030-3220407 [Herbaspirillum rubrisubalbicans M1]
MFTNEKPLEWIASSYKDLMALPAEVRQRFGFALSLAQMGERDDTAKVLKGFGGAGVLEVVEYQMGNAYRAVSTVKFEEAV
FVLHCFQKKSKSGIATPHTEMDIVRTRLNVAAARAEELKHAKKNR

Nucleotide

• Length: 378 bp

>T59243 NZ_CP013737:3220030-3220407 [Herbaspirillum rubrisubalbicans M1]
ATGTTTACCAATGAGAAACCGCTCGAATGGATTGCGAGCAGCTACAAGGATCTGATGGCGCTGCCTGCGGAGGTTCGTCA
GCGTTTCGGCTTTGCCCTGTCGCTTGCCCAGATGGGAGAGCGCGACGATACGGCCAAGGTCTTGAAAGGTTTCGGCGGTG
CCGGGGTATTGGAAGTTGTCGAATACCAAATGGGCAATGCCTACCGCGCGGTCTCCACGGTGAAATTCGAAGAAGCGGTG
TTCGTGCTGCATTGCTTTCAGAAAAAGAGCAAAAGCGGCATCGCCACTCCCCACACGGAGATGGACATCGTTCGGACACG
TCTCAACGTGGCCGCAGCAAGAGCCGAGGAATTGAAACATGCAAAAAAGAATCGTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12286.51 Da
• Isoelectric Point: 10.4695

>AT59243 WP_017450286.1 NZ_CP013737:3220388-3220723 [Herbaspirillum rubrisubalbicans M1]
MQKRIVEETEVLRSTSNVFADLGLPQADKLRIKTGLVIEIRKAMRALGLTQQAAAQRMGIPQPKVSGMMRGDFSNLSERK
LMDCLNRLGYDIDIRVCPATATVGQLKLTVV

Nucleotide

• Length: 336 bp

>AT59243 NZ_CP013737:3220388-3220723 [Herbaspirillum rubrisubalbicans M1]
ATGCAAAAAAGAATCGTTGAAGAAACCGAAGTCCTTCGCAGCACCAGCAACGTCTTCGCGGACCTGGGATTGCCACAGGC
CGACAAGCTGCGCATCAAGACCGGGCTGGTGATCGAAATCCGCAAGGCCATGCGCGCCCTTGGACTTACCCAGCAAGCGG
CGGCACAGCGCATGGGGATTCCGCAACCGAAGGTGTCGGGCATGATGCGCGGTGACTTCAGCAATCTTTCCGAGCGCAAG
TTAATGGATTGTCTGAATCGACTGGGTTATGACATCGATATCCGGGTGTGCCCCGCTACCGCTACGGTAGGACAATTGAA
GCTCACGGTCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A0U3LK25

Antitoxin

• AlphaFold DB: A0A0U3CND7