Detailed information of TA system TA059231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1185776..1187416
• Replicon: chromosome
• Accession: NZ_CP013729
• Organism: Roseateles depolymerans strain KCTC 42856

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0U3MDJ1
• Locus tag: RD2015_RS05215
• Protein ID: WP_058933998.1
• Coordinates: 1185776..1187125 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: RD2015_RS24225
• Protein ID: WP_083525354.1
• Coordinates: 1187129..1187416 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RD2015_RS05190	1182003..1183289	+	1287	WP_198164902.1	putative Na+/H+ antiporter	-
RD2015_RS05195	1183276..1184304	-	1029	WP_058933994.1	hypothetical protein	-
RD2015_RS05200	1184435..1184647	-	213	WP_058933995.1	cold-shock protein	-
RD2015_RS05205	1184883..1185236	-	354	WP_058933996.1	RNA-binding protein	-
RD2015_RS05210	1185310..1185504	-	195	WP_058933997.1	hypothetical protein	-
RD2015_RS05215	1185776..1187125	-	1350	WP_058933998.1	type II toxin-antitoxin system HipA family toxin	Toxin
RD2015_RS24225	1187129..1187416	-	288	WP_083525354.1	helix-turn-helix domain-containing protein	Antitoxin
RD2015_RS05225	1187844..1188773	-	930	WP_058933999.1	polyprenyl synthetase family protein	-
RD2015_RS05230	1189457..1189768	+	312	WP_058934000.1	50S ribosomal protein L21	-
RD2015_RS05235	1189819..1190076	+	258	WP_058934001.1	50S ribosomal protein L27	-
RD2015_RS05240	1190267..1191310	+	1044	Protein_1051	GTPase ObgE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-414)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49968.13 Da
• Isoelectric Point: 6.9611

>T59231 WP_058933998.1 NZ_CP013729:c1187125-1185776 [Roseateles depolymerans]
MGRRSHSRTLGLWMNGAPVGRWTLAPNAPDTLQYDLAWTQSSQGRPLSLSLPFTPGNTPHHGEKVRAFFENLLPDSKDIR
ERLARRFATGSTDAFELLAEIGRDCVGALEILPEDQTSTGIAPLQAEALDESRVAQLLRNTTRPGLLSRGDEDDEFRISI
AGAQEKTALLLHDGQWCLPHGNTPTTHIFKLPLGLIGGMKLDMRHSVENEWLCALILKAFGLPVAPCWPAQFEDMKVLVV
ERFDRAWWTPSSGDTRLIRLPQEDMCQATGVSPDNKYEADGGPGMDQILDLLNGSITREQDRRHFFTAQLLFWMLCATDG
HAKNFSLFLRPGGRFQLTPLYDVLSAYPVLGAGPAKMSPHKVKMAMAVRSKNAHWKMRDILRRHWVALGERHGILTEGGR
EVAYLIDEVVAQTPRVIATVRTLLPNGFPETVANSLFEGLQSAADQLRQ

Nucleotide

• Length: 1350 bp

>T59231 NZ_CP013729:c1187125-1185776 [Roseateles depolymerans]
ATGGGACGCCGCTCACACTCCCGGACCCTCGGTCTCTGGATGAACGGGGCCCCTGTCGGCCGTTGGACACTGGCACCGAA
CGCCCCCGACACACTCCAATACGACCTTGCCTGGACTCAGTCCTCACAGGGGCGCCCTTTGTCCCTGTCTTTGCCATTCA
CGCCCGGCAACACGCCACACCATGGCGAGAAGGTCCGCGCTTTTTTCGAGAACCTGCTGCCGGACAGCAAAGACATCCGC
GAGCGCCTGGCTCGCCGCTTCGCCACCGGGTCGACCGACGCTTTCGAGTTGCTTGCCGAGATTGGCCGGGACTGCGTCGG
GGCACTGGAGATCCTGCCTGAGGACCAGACCTCCACGGGCATCGCCCCTTTGCAGGCGGAAGCTCTGGATGAGAGCCGGG
TCGCCCAACTGCTGCGCAACACGACTCGCCCAGGCCTCTTGAGTCGCGGTGACGAAGACGACGAATTCCGAATCTCCATC
GCCGGGGCTCAAGAAAAAACGGCCCTGCTGCTTCACGACGGCCAGTGGTGTTTGCCGCACGGCAATACGCCGACCACCCA
TATCTTCAAACTTCCCCTGGGGCTGATCGGCGGCATGAAGCTCGACATGCGCCATTCGGTGGAGAACGAGTGGCTTTGCG
CCTTGATTCTCAAAGCGTTTGGTCTGCCCGTCGCACCTTGCTGGCCCGCGCAATTCGAGGACATGAAGGTGCTGGTCGTC
GAACGATTCGACCGCGCTTGGTGGACGCCGTCCTCTGGAGACACTCGCCTGATCCGCCTGCCGCAAGAGGACATGTGCCA
GGCCACGGGTGTCTCGCCGGACAACAAATACGAAGCCGACGGCGGCCCCGGTATGGACCAGATCCTTGACCTGTTGAATG
GCTCCATCACCCGTGAGCAGGACAGGCGCCACTTCTTTACCGCCCAGCTGCTGTTCTGGATGCTGTGCGCCACCGACGGC
CACGCCAAGAACTTCAGCCTGTTCCTGCGTCCTGGAGGACGCTTCCAACTCACGCCGCTGTACGACGTCCTGTCCGCCTA
CCCGGTGCTTGGCGCAGGCCCTGCAAAGATGTCGCCGCACAAGGTCAAGATGGCCATGGCGGTGCGCTCCAAAAATGCGC
ACTGGAAGATGCGAGACATCCTGCGACGCCACTGGGTCGCCCTGGGCGAGCGGCACGGCATCTTGACCGAAGGCGGGCGT
GAGGTGGCGTATCTGATCGACGAGGTGGTGGCTCAAACGCCACGGGTCATCGCCACCGTGCGCACCCTGCTGCCGAATGG
GTTTCCGGAGACCGTCGCCAACAGCCTCTTCGAGGGCCTGCAGAGCGCGGCTGACCAGCTTCGCCAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10241.62 Da
• Isoelectric Point: 10.0849

>AT59231 WP_083525354.1 NZ_CP013729:c1187416-1187129 [Roseateles depolymerans]
MSQSFTVRTADQLTTLLQAFRKDAGLTQSQVAQRLGVTQQTYSALERNAATVGAARLLKLLHILGVELALSKRDPDASPV
PPPGPTRSGTEQPDW

Nucleotide

• Length: 288 bp

>AT59231 NZ_CP013729:c1187416-1187129 [Roseateles depolymerans]
ATGAGCCAATCTTTCACCGTCCGCACGGCTGATCAACTGACGACCTTGCTGCAGGCATTTCGAAAGGATGCTGGGCTGAC
CCAGAGCCAGGTGGCTCAGCGTCTTGGCGTCACACAGCAAACTTACTCTGCGTTGGAACGCAACGCCGCAACGGTCGGCG
CGGCTCGGCTGCTGAAGCTGCTGCACATCCTTGGCGTTGAGCTGGCACTCAGCAAACGCGATCCCGATGCATCACCTGTC
CCCCCTCCAGGGCCCACACGGTCTGGCACCGAACAGCCTGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0U3MDJ1