Detailed information of TA system TA059230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3366554..3367191
• Replicon: chromosome
• Accession: NZ_CP013727
• Organism: Bacillus amyloliquefaciens strain MBE1283

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AVM03_RS16200
• Protein ID: WP_003156187.1
• Coordinates: 3366841..3367191 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AVM03_RS16195
• Protein ID: WP_003156188.1
• Coordinates: 3366554..3366835 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVM03_RS16175	3362920..3363519	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
AVM03_RS16180	3363612..3363977	+	366	WP_014304402.1	holo-ACP synthase	-
AVM03_RS16185	3364141..3365148	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
AVM03_RS16190	3365265..3366434	+	1170	WP_058906584.1	alanine racemase	-
AVM03_RS16195	3366554..3366835	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AVM03_RS16200	3366841..3367191	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AVM03_RS16205	3367309..3368130	+	822	WP_014304404.1	STAS domain-containing protein	-
AVM03_RS16210	3368135..3368500	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AVM03_RS16215	3368503..3368904	+	402	WP_003156178.1	anti-sigma regulatory factor	-
AVM03_RS16220	3368916..3369923	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
AVM03_RS16225	3369987..3370316	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AVM03_RS16230	3370313..3370795	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
AVM03_RS16235	3370761..3371549	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
AVM03_RS16240	3371549..3372151	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T59230 WP_003156187.1 NZ_CP013727:3366841-3367191 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T59230 NZ_CP013727:3366841-3367191 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT59230 WP_003156188.1 NZ_CP013727:3366554-3366835 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT59230 NZ_CP013727:3366554-3366835 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5