Detailed information of TA system TA059227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1825035..1825564
• Replicon: chromosome
• Accession: NZ_CP013714
• Organism: Staphylococcus equorum strain C2014

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S376
• Locus tag: AVJ22_RS08895
• Protein ID: WP_002511418.1
• Coordinates: 1825035..1825397 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J9GY23
• Locus tag: AVJ22_RS08900
• Protein ID: WP_002507108.1
• Coordinates: 1825394..1825564 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVJ22_RS08875	1822040..1822810	-	771	WP_002507114.1	RNA polymerase sigma factor SigB	-
AVJ22_RS08880	1822785..1823267	-	483	WP_065338422.1	anti-sigma B factor RsbW	-
AVJ22_RS08885	1823269..1823595	-	327	WP_002511417.1	anti-sigma factor antagonist	-
AVJ22_RS08890	1823675..1824676	-	1002	WP_065338423.1	PP2C family protein-serine/threonine phosphatase	-
AVJ22_RS08895	1825035..1825397	-	363	WP_002511418.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVJ22_RS08900	1825394..1825564	-	171	WP_002507108.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AVJ22_RS08905	1825652..1826800	-	1149	WP_065338424.1	alanine racemase	-
AVJ22_RS08910	1826923..1827273	-	351	WP_065338425.1	holo-ACP synthase	-
AVJ22_RS08915	1827306..1827842	-	537	WP_065338426.1	PH domain-containing protein	-
AVJ22_RS08920	1827835..1829340	-	1506	WP_065338427.1	PH domain-containing protein	-
AVJ22_RS08925	1829333..1829812	-	480	WP_065338428.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13432.59 Da
• Isoelectric Point: 10.2178

>T59227 WP_002511418.1 NZ_CP013714:c1825397-1825035 [Staphylococcus equorum]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSESKMKEVNVAIDISLGLHNVRNSKS

Nucleotide

• Length: 363 bp

>T59227 NZ_CP013714:c1825397-1825035 [Staphylococcus equorum]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATCATACAGAATGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAGCTTGATAAAGATTCTGTAATACTGCTTGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACATACCTTTCTGAAAGTAAAATGAAAGAAGTTAATGTTGCCATAGA
TATAAGCTTAGGATTACATAATGTTAGAAATAGTAAATCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT59227 WP_002507108.1 NZ_CP013714:c1825564-1825394 [Staphylococcus equorum]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYLSSKNNNE

Nucleotide

• Length: 171 bp

>AT59227 NZ_CP013714:c1825564-1825394 [Staphylococcus equorum]
ATGGCAAGTTTTAATCAAAGCAGAAACCATAGTATTGAACAACTATTAAAAGAAGGCTATTCTCAAATGGCCGATTTAAA
TCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACCTCAGCTCTAAGAATA
ACAATGAATGA

Structures

Toxin

• AlphaFold DB: K7S376

Antitoxin

• AlphaFold DB: J9GY23