Detailed information of TA system TA059225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 42445..43355
• Replicon: plasmid 2
• Accession: NZ_CP013713
• Organism: Klebsiella pneumoniae strain J1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AU361_RS26800
• Protein ID: WP_032413375.1
• Coordinates: 42885..43355 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: AU361_RS26795
• Protein ID: WP_032413443.1
• Coordinates: 42445..42888 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU361_RS26770	38279..39277	+	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-
AU361_RS26775	39324..40502	+	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
AU361_RS26780	40639..40986	+	348	WP_032413377.1	hypothetical protein	-
AU361_RS26785	41044..41169	+	126	Protein_44	transposase	-
AU361_RS28400	41303..41527	-	225	Protein_45	transposase	-
AU361_RS28405	41547..41732	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
AU361_RS26790	41833..42345	+	513	Protein_47	DUF4113 domain-containing protein	-
AU361_RS26795	42445..42888	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
AU361_RS26800	42885..43355	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
AU361_RS26805	43465..43725	-	261	WP_017901409.1	hypothetical protein	-
AU361_RS28410	44297..44674	-	378	Protein_51	transposase	-
AU361_RS26815	44808..45776	+	969	WP_077265492.1	IS5 family transposase	-
AU361_RS26820	45834..46883	-	1050	Protein_53	IS66 family transposase	-
AU361_RS26825	46932..47279	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
AU361_RS26830	47276..47614	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
AU361_RS26835	47707..48165	+	459	Protein_56	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53400	53400
	-	inside	IScluster/Tn	-	-	44853..50210	5357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T59225 WP_032413375.1 NZ_CP013713:42885-43355 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T59225 NZ_CP013713:42885-43355 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT59225 WP_032413443.1 NZ_CP013713:42445-42888 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT59225 NZ_CP013713:42445-42888 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4