Detailed information of TA system TA059224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 50775..51526
• Replicon: plasmid 1
• Accession: NZ_CP013712
• Organism: Klebsiella pneumoniae strain J1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AU361_RS26460
• Protein ID: WP_049013153.1
• Coordinates: 51044..51526 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: AU361_RS26455
• Protein ID: WP_004902250.1
• Coordinates: 50775..51053 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU361_RS26435	46733..48288	-	1556	Protein_55	IS66 family transposase	-
AU361_RS26440	48350..48700	-	351	WP_004200420.1	IS66 family insertion sequence element accessory protein TnpB	-
AU361_RS28280	48697..49104	-	408	WP_077259088.1	transposase	-
AU361_RS26445	49203..49943	+	741	WP_049013155.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
AU361_RS26450	50441..50653	+	213	WP_016946198.1	hypothetical protein	-
AU361_RS26455	50775..51053	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
AU361_RS26460	51044..51526	+	483	WP_049013153.1	GNAT family N-acetyltransferase	Toxin
AU361_RS28290	51636..52072	-	437	Protein_62	IS1 family transposase	-
AU361_RS28295	52155..52559	+	405	WP_071888099.1	IS66 family insertion sequence hypothetical protein	-
AU361_RS26470	52556..52903	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
AU361_RS26475	52952..54496	+	1545	WP_058837173.1	IS66 family transposase	-
AU361_RS28300	54513..54788	-	276	Protein_66	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..74973	74973
	-	inside	IScluster/Tn	formA	-	39727..67860	28133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17652.36 Da
• Isoelectric Point: 8.6269

>T59224 WP_049013153.1 NZ_CP013712:51044-51526 [Klebsiella pneumoniae]
MGMRAPESLTSEHNIAEFCCQEPALNEWLKKKALRNNSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAAILKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTHFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T59224 NZ_CP013712:51044-51526 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAGTCCCTGACGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACAACAGTACTGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTTATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCAATTCT
TAAGGATGCTATCTACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCATTTTGGCTTTGAACCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT59224 WP_004902250.1 NZ_CP013712:50775-51053 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT59224 NZ_CP013712:50775-51053 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTCATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3