Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4166072..4166703 Replicon chromosome
Accession NZ_CP013663
Organism Escherichia coli strain GB089

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag CO57_RS22650 Protein ID WP_001259388.1
Coordinates 4166428..4166703 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CO57_RS22645 Protein ID WP_000593555.1
Coordinates 4166072..4166431 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO57_RS22620 4162204..4163148 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
CO57_RS22625 4163145..4163978 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CO57_RS22630 4163978..4164742 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
CO57_RS22635 4164739..4165545 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
CO57_RS22640 4165551..4165952 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CO57_RS22645 4166072..4166431 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CO57_RS22650 4166428..4166703 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
CO57_RS22655 4166776..4167900 - 1125 WP_001314210.1 ABC transporter permease -
CO57_RS22660 4167900..4170635 - 2736 WP_033817337.1 ribosome-associated ATPase/putative transporter RbbA -
CO57_RS22665 4170632..4171699 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T59161 WP_001259388.1 NZ_CP013663:c4166703-4166428 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T59161 NZ_CP013663:c4166703-4166428 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT59161 WP_000593555.1 NZ_CP013663:c4166431-4166072 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT59161 NZ_CP013663:c4166431-4166072 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References