Detailed information of TA system TA059158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3107300..3107925
• Replicon: chromosome
• Accession: NZ_CP013663
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CO57_RS17225
• Protein ID: WP_000911330.1
• Coordinates: 3107300..3107698 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CO57_RS17230
• Protein ID: WP_000450524.1
• Coordinates: 3107698..3107925 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS17205	3103178..3103378	+	201	WP_000383836.1	YpfN family protein	-
CO57_RS17210	3103488..3104186	-	699	WP_000679823.1	esterase	-
CO57_RS17215	3104260..3106275	-	2016	WP_032197281.1	tRNA cytosine(34) acetyltransferase TmcA	-
CO57_RS17220	3106290..3107153	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
CO57_RS17225	3107300..3107698	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CO57_RS17230	3107698..3107925	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CO57_RS17235	3108079..3108792	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CO57_RS17245	3109005..3110039	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
CO57_RS17250	3110056..3110934	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CO57_RS17255	3111080..3111652	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CO57_RS17260	3111652..3112122	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T59158 WP_000911330.1 NZ_CP013663:c3107698-3107300 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T59158 NZ_CP013663:c3107698-3107300 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT59158 WP_000450524.1 NZ_CP013663:c3107925-3107698 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT59158 NZ_CP013663:c3107925-3107698 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33