Detailed information of TA system TA059149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1974876..1975402
• Replicon: chromosome
• Accession: NZ_CP013663
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CO57_RS11285
• Protein ID: WP_000323025.1
• Coordinates: 1974876..1975163 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CO57_RS11290
• Protein ID: WP_000534858.1
• Coordinates: 1975163..1975402 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS30915	1970553..1970684	-	132	WP_047626566.1	DUF3927 family protein	-
CO57_RS11255	1971580..1972401	-	822	WP_000762885.1	antitermination protein	-
CO57_RS11260	1972416..1972772	-	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
CO57_RS11265	1972785..1973834	-	1050	WP_053292618.1	DUF968 domain-containing protein	-
CO57_RS11270	1973836..1974114	-	279	WP_012304870.1	hypothetical protein	-
CO57_RS11275	1974181..1974432	-	252	WP_000980994.1	hypothetical protein	-
CO57_RS11280	1974649..1974804	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CO57_RS11285	1974876..1975163	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CO57_RS11290	1975163..1975402	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CO57_RS28755	1975427..1975732	+	306	WP_071527819.1	hypothetical protein	-
CO57_RS11295	1975935..1976267	+	333	WP_001317460.1	FlxA-like family protein	-
CO57_RS11300	1976704..1978044	-	1341	WP_000589012.1	ISNCY family transposase	-
CO57_RS11305	1978078..1978497	-	420	WP_001151195.1	DUF977 family protein	-
CO57_RS11310	1978538..1979503	-	966	WP_000054504.1	hypothetical protein	-
CO57_RS11315	1979484..1980005	-	522	WP_000705349.1	hypothetical protein	-
CO57_RS11320	1979989..1980216	-	228	WP_000476993.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1938554..1998449	59895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T59149 WP_000323025.1 NZ_CP013663:c1975163-1974876 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T59149 NZ_CP013663:c1975163-1974876 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT59149 WP_000534858.1 NZ_CP013663:c1975402-1975163 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT59149 NZ_CP013663:c1975402-1975163 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8