Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4119745..4120376 Replicon chromosome
Accession NZ_CP013662
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag CP48_RS22350 Protein ID WP_001259388.1
Coordinates 4120101..4120376 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CP48_RS22345 Protein ID WP_000593555.1
Coordinates 4119745..4120104 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS22320 4115877..4116821 + 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -
CP48_RS22325 4116818..4117651 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CP48_RS22330 4117651..4118415 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
CP48_RS22335 4118412..4119218 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
CP48_RS22340 4119224..4119625 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CP48_RS22345 4119745..4120104 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CP48_RS22350 4120101..4120376 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
CP48_RS22355 4120449..4121573 - 1125 WP_001216257.1 ABC transporter permease -
CP48_RS22360 4121573..4124308 - 2736 WP_032294472.1 ribosome-associated ATPase/putative transporter RbbA -
CP48_RS22365 4124305..4125372 - 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T59138 WP_001259388.1 NZ_CP013662:c4120376-4120101 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T59138 NZ_CP013662:c4120376-4120101 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATTGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT59138 WP_000593555.1 NZ_CP013662:c4120104-4119745 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT59138 NZ_CP013662:c4120104-4119745 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References