Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3462720..3463374 Replicon chromosome
Accession NZ_CP013662
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CP48_RS18975 Protein ID WP_000244781.1
Coordinates 3462720..3463127 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CP48_RS18980 Protein ID WP_000354046.1
Coordinates 3463108..3463374 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS18955 3458677..3460410 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
CP48_RS18960 3460416..3461126 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CP48_RS18965 3461151..3462047 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CP48_RS18970 3462159..3462680 + 522 WP_001055874.1 flavodoxin FldB -
CP48_RS18975 3462720..3463127 - 408 WP_000244781.1 protein YgfX Toxin
CP48_RS18980 3463108..3463374 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CP48_RS18985 3463617..3464597 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
CP48_RS18990 3464793..3465452 - 660 WP_000250274.1 hemolysin III family protein -
CP48_RS18995 3465616..3465927 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CP48_RS19000 3465972..3467405 + 1434 WP_040090423.1 6-phospho-beta-glucosidase BglA -
CP48_RS19005 3467462..3468205 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T59134 WP_000244781.1 NZ_CP013662:c3463127-3462720 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T59134 NZ_CP013662:c3463127-3462720 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCTTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT59134 WP_000354046.1 NZ_CP013662:c3463374-3463108 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT59134 NZ_CP013662:c3463374-3463108 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References