Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1699665..1700303 Replicon chromosome
Accession NZ_CP013662
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CP48_RS10140 Protein ID WP_000813794.1
Coordinates 1699665..1699841 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CP48_RS10145 Protein ID WP_001270286.1
Coordinates 1699887..1700303 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS10120 1695284..1696498 - 1215 WP_001326689.1 BenE family transporter YdcO -
CP48_RS10125 1696551..1697087 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CP48_RS10130 1697160..1699121 + 1962 WP_001409409.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CP48_RS10135 1699213..1699443 - 231 WP_000494244.1 YncJ family protein -
CP48_RS10140 1699665..1699841 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CP48_RS10145 1699887..1700303 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CP48_RS10150 1700382..1701788 + 1407 WP_040090576.1 PLP-dependent aminotransferase family protein -
CP48_RS10155 1702033..1703178 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CP48_RS10160 1703196..1704209 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
CP48_RS10165 1704210..1705151 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T59121 WP_000813794.1 NZ_CP013662:1699665-1699841 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T59121 NZ_CP013662:1699665-1699841 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT59121 WP_001270286.1 NZ_CP013662:1699887-1700303 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT59121 NZ_CP013662:1699887-1700303 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References