Detailed information of TA system TA059120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1490822..1491303
• Replicon: chromosome
• Accession: NZ_CP013662
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0B1KMH3
• Locus tag: CP48_RS09040
• Protein ID: WP_042966241.1
• Coordinates: 1490822..1491112 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0B1KNA0
• Locus tag: CP48_RS09045
• Protein ID: WP_042966240.1
• Coordinates: 1491112..1491303 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS09010	1488017..1488220	-	204	WP_042966247.1	DUF1482 family protein	-
CP48_RS09015	1488217..1488405	-	189	WP_000449183.1	cell division inhibition protein DicB	-
CP48_RS28745	1488620..1488883	+	264	WP_001353256.1	DUF1883 domain-containing protein	-
CP48_RS09020	1488824..1489045	-	222	WP_042966246.1	hypothetical protein	-
CP48_RS09025	1489410..1489619	+	210	WP_042966244.1	hypothetical protein	-
CP48_RS09030	1489620..1490258	-	639	WP_042966243.1	hypothetical protein	-
CP48_RS09035	1490400..1490555	-	156	WP_052318772.1	DUF1391 family protein	-
CP48_RS09040	1490822..1491112	-	291	WP_042966241.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP48_RS09045	1491112..1491303	-	192	WP_042966240.1	hypothetical protein	Antitoxin
CP48_RS09050	1491321..1491821	-	501	WP_033812093.1	helix-turn-helix transcriptional regulator	-
CP48_RS09055	1491929..1492204	+	276	WP_042966239.1	helix-turn-helix domain-containing protein	-
CP48_RS09060	1492188..1492613	+	426	WP_047088917.1	Rha family transcriptional regulator	-
CP48_RS31325	1492685..1493719	+	1035	WP_047088918.1	pyocin large subunit	-
CP48_RS31330	1493712..1494173	+	462	WP_157845346.1	replication protein	-
CP48_RS09075	1494208..1494984	+	777	WP_047088919.1	DUF1627 domain-containing protein	-
CP48_RS09080	1495000..1495422	+	423	WP_047088920.1	DUF977 family protein	-
CP48_RS09085	1495446..1495907	+	462	WP_187231401.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1479909..1534431	54522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11206.07 Da
• Isoelectric Point: 9.9854

>T59120 WP_042966241.1 NZ_CP013662:c1491112-1490822 [Escherichia coli]
MLPILWLPSAREDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTTSNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T59120 NZ_CP013662:c1491112-1490822 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGAGGATTTACGTCAGATAGTAGCCTATATTGCTAAAGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGAGAAATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAACATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGGCAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6987.80 Da
• Isoelectric Point: 4.0270

>AT59120 WP_042966240.1 NZ_CP013662:c1491303-1491112 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT59120 NZ_CP013662:c1491303-1491112 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACGGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAGC
CAAAGTGGCAGCAAGCCTCGCAGATCCCCGTCCTGCAATTCCGCATGATGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0B1KMH3

Antitoxin

• AlphaFold DB: A0A0B1KNA0