Detailed information of TA system TA059115

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1067733..1068528
• Replicon: chromosome
• Accession: NZ_CP013662
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: CP48_RS06810
• Protein ID: WP_000854914.1
• Coordinates: 1068154..1068528 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: CP48_RS06805
• Protein ID: WP_001280954.1
• Coordinates: 1067733..1068107 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS06765	1063088..1063993	+	906	WP_000203541.1	chemotaxis protein	-
CP48_RS06770	1063990..1065060	+	1071	WP_065225012.1	patatin-like phospholipase family protein	-
CP48_RS06780	1065400..1066218	+	819	WP_001234682.1	DUF945 domain-containing protein	-
CP48_RS06785	1066309..1066794	+	486	WP_000214398.1	antirestriction protein	-
CP48_RS06790	1066810..1067286	+	477	WP_001186738.1	RadC family protein	-
CP48_RS06795	1067349..1067570	+	222	WP_047088896.1	DUF987 domain-containing protein	-
CP48_RS06805	1067733..1068107	+	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CP48_RS06810	1068154..1068528	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
CP48_RS06815	1068525..1069016	+	492	WP_000976857.1	hypothetical protein	-
CP48_RS06820	1069028..1069225	+	198	WP_000839282.1	DUF957 domain-containing protein	-
CP48_RS06825	1069310..1070152	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
CP48_RS06835	1070642..1070860	-	219	WP_001040187.1	translation initiation factor IF-1	-
CP48_RS06840	1071145..1071849	-	705	WP_001241677.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T59115 WP_000854914.1 NZ_CP013662:1068154-1068528 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T59115 NZ_CP013662:1068154-1068528 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT59115 WP_001280954.1 NZ_CP013662:1067733-1068107 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT59115 NZ_CP013662:1067733-1068107 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41