Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 221355..221994 Replicon chromosome
Accession NZ_CP013659
Organism Planococcus rifietoensis strain M8

Toxin (Protein)


Gene name pemK Uniprot ID A0A1C7EC77
Locus tag AUC31_RS01105 Protein ID WP_058381776.1
Coordinates 221644..221994 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1C7EBE9
Locus tag AUC31_RS01100 Protein ID WP_058381777.1
Coordinates 221355..221639 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AUC31_RS01075 217058..217537 + 480 WP_058381781.1 PH domain-containing protein -
AUC31_RS01080 217530..219092 + 1563 WP_058383705.1 PH domain-containing protein -
AUC31_RS01085 219109..219723 - 615 WP_058381780.1 rhomboid family intramembrane serine protease -
AUC31_RS01090 219792..220145 + 354 WP_058381779.1 holo-ACP synthase -
AUC31_RS01095 220162..221310 + 1149 WP_058381778.1 alanine racemase -
AUC31_RS01100 221355..221639 + 285 WP_058381777.1 hypothetical protein Antitoxin
AUC31_RS01105 221644..221994 + 351 WP_058381776.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AUC31_RS01110 222173..223003 + 831 WP_058381775.1 STAS domain-containing protein -
AUC31_RS01115 223000..223362 + 363 WP_058381774.1 STAS domain-containing protein -
AUC31_RS01120 223365..223766 + 402 WP_058381773.1 anti-sigma regulatory factor -
AUC31_RS01125 223779..224780 + 1002 WP_058381772.1 PP2C family protein-serine/threonine phosphatase -
AUC31_RS01130 224876..225208 + 333 WP_058381771.1 anti-sigma factor antagonist -
AUC31_RS01135 225211..225681 + 471 WP_058381770.1 anti-sigma B factor RsbW -
AUC31_RS01140 225659..226441 + 783 WP_058381769.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12850.94 Da        Isoelectric Point: 5.8817

>T59111 WP_058381776.1 NZ_CP013659:221644-221994 [Planococcus rifietoensis]
MIVKRGDVFFAELSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDDALMEKVDEALEISVGLVKF

Download         Length: 351 bp

>T59111 NZ_CP013659:221644-221994 [Planococcus rifietoensis]
TTGATTGTAAAACGCGGGGATGTTTTTTTCGCAGAATTATCACCGGTCGTCGGGTCTGAGCAAGGCGGGACCCGTCCGGT
CTTGGTGATACAAAACGATATCGGGAACCGGTTTAGTCCCACAGTGATCATTGCGGCAATCACCGCCCAGATCCAGAAAG
CCAAATTGCCGACCCACGTGGAAATCAACGCCAAAAAATACGGCTTTGAGCGTGATTCTGTTATTTTGCTTGAACAATTG
CGGACGATTGATAAATCGCGTTTGACCGATAAGATTACACAGCTGGACGATGCGTTGATGGAAAAGGTGGACGAAGCCTT
GGAAATCAGTGTCGGACTTGTAAAGTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10763.11 Da        Isoelectric Point: 5.1572

>AT59111 WP_058381777.1 NZ_CP013659:221355-221639 [Planococcus rifietoensis]
VYEKSKTKEVVVKLPKQFMSELTAHSDERIEENGEFIYVSTQRVTKNVYDSYHIREAMIKGYVEMSQINLSIACECSHAE
YEAEHTTVQLVSGG

Download         Length: 285 bp

>AT59111 NZ_CP013659:221355-221639 [Planococcus rifietoensis]
GTGTACGAGAAGTCAAAGACGAAAGAAGTGGTAGTCAAGTTGCCGAAGCAATTCATGTCGGAGCTTACTGCCCATTCAGA
TGAACGCATTGAAGAGAACGGTGAATTCATCTACGTATCCACTCAACGGGTAACAAAAAATGTCTACGATTCATATCATA
TTCGGGAAGCGATGATCAAGGGCTACGTGGAAATGTCGCAGATCAACCTGTCGATTGCCTGCGAATGTTCGCACGCTGAG
TACGAAGCGGAGCACACGACAGTGCAACTCGTAAGCGGAGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C7EC77

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7EBE9

References