Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4208573..4209093 Replicon chromosome
Accession NZ_CP013658
Organism Escherichia coli strain uk_P46212

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag AUO99_RS22030 Protein ID WP_001160964.1
Coordinates 4208809..4209093 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AUO99_RS22025 Protein ID WP_000125566.1
Coordinates 4208573..4208806 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AUO99_RS22000 4203646..4203933 + 288 WP_000203744.1 ferredoxin-like protein FixX -
AUO99_RS22005 4203992..4205323 + 1332 WP_001183209.1 MFS transporter -
AUO99_RS22010 4205431..4205961 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AUO99_RS22015 4205954..4207816 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
AUO99_RS22020 4208008..4208487 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AUO99_RS22025 4208573..4208806 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AUO99_RS22030 4208809..4209093 + 285 WP_001160964.1 CcdB family protein Toxin
AUO99_RS22035 4209120..4209968 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AUO99_RS22040 4209975..4210352 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AUO99_RS22045 4210355..4211176 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AUO99_RS22050 4211173..4212162 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AUO99_RS22055 4212162..4213448 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T59107 WP_001160964.1 NZ_CP013658:4208809-4209093 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T59107 NZ_CP013658:4208809-4209093 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT59107 WP_000125566.1 NZ_CP013658:4208573-4208806 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT59107 NZ_CP013658:4208573-4208806 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References