Detailed information of TA system TA059104

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4017469..4018304
• Replicon: chromosome
• Accession: NZ_CP013658
• Organism: Escherichia coli strain uk_P46212

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: AUO99_RS21060
• Protein ID: WP_000854759.1
• Coordinates: 4017927..4018304 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AUO99_RS21055
• Protein ID: WP_001295723.1
• Coordinates: 4017469..4017837 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO99_RS21025	4013471..4013944	+	474	WP_001350782.1	antirestriction protein	-
AUO99_RS21030	4013960..4014436	+	477	WP_001186775.1	RadC family protein	-
AUO99_RS21035	4014499..4014753	+	255	WP_023281719.1	DUF987 domain-containing protein	-
AUO99_RS21040	4014894..4016435	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
AUO99_RS21045	4016450..4017196	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
AUO99_RS21055	4017469..4017837	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AUO99_RS21060	4017927..4018304	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
AUO99_RS21065	4018301..4018789	+	489	WP_000761690.1	hypothetical protein	-
AUO99_RS21070	4018806..4018982	+	177	WP_000839286.1	DUF957 domain-containing protein	-
AUO99_RS29620	4019082..4019240	+	159	WP_001467148.1	hypothetical protein	-
AUO99_RS29475	4019604..4019780	+	177	Protein_3840	helix-turn-helix domain-containing protein	-
AUO99_RS21080	4020553..4022193	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T59104 WP_000854759.1 NZ_CP013658:4017927-4018304 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T59104 NZ_CP013658:4017927-4018304 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT59104 WP_001295723.1 NZ_CP013658:4017469-4017837 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT59104 NZ_CP013658:4017469-4017837 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52