Detailed information of TA system TA059100

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3242574..3243409
• Replicon: chromosome
• Accession: NZ_CP013658
• Organism: Escherichia coli strain uk_P46212

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A376WVB1
• Locus tag: AUO99_RS17275
• Protein ID: WP_001094448.1
• Coordinates: 3243032..3243409 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3T7EC32
• Locus tag: AUO99_RS17270
• Protein ID: WP_024175935.1
• Coordinates: 3242574..3242942 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO99_RS17245	3240003..3240236	+	234	WP_001117568.1	DUF905 family protein	-
AUO99_RS17250	3240327..3241145	+	819	WP_001234753.1	DUF945 domain-containing protein	-
AUO99_RS17255	3241237..3241722	+	486	WP_000214415.1	antirestriction protein	-
AUO99_RS17260	3241734..3242210	+	477	WP_001186709.1	RadC family protein	-
AUO99_RS17265	3242279..3242500	+	222	WP_000692346.1	DUF987 domain-containing protein	-
AUO99_RS17270	3242574..3242942	+	369	WP_024175935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AUO99_RS17275	3243032..3243409	+	378	WP_001094448.1	TA system toxin CbtA family protein	Toxin
AUO99_RS17280	3243406..3243894	+	489	WP_000761714.1	hypothetical protein	-
AUO99_RS17285	3243906..3244127	+	222	WP_001296561.1	DUF957 domain-containing protein	-
AUO99_RS17290	3244183..3245028	+	846	WP_000065751.1	DUF4942 domain-containing protein	-
AUO99_RS17300	3245621..3246118	+	498	WP_000509815.1	hypothetical protein	-
AUO99_RS17305	3246296..3247219	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
AUO99_RS17310	3247223..3248041	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3235043..3246118	11075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14221.22 Da
• Isoelectric Point: 8.2919

>T59100 WP_001094448.1 NZ_CP013658:3243032-3243409 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T59100 NZ_CP013658:3243032-3243409 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13598.40 Da
• Isoelectric Point: 6.6255

>AT59100 WP_024175935.1 NZ_CP013658:3242574-3242942 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT59100 NZ_CP013658:3242574-3242942 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A376WVB1

Antitoxin

• AlphaFold DB: A0A3T7EC32