Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2256631..2257285 Replicon chromosome
Accession NZ_CP013658
Organism Escherichia coli strain uk_P46212

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag AUO99_RS12310 Protein ID WP_000244765.1
Coordinates 2256631..2257038 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag AUO99_RS12315 Protein ID WP_000354050.1
Coordinates 2257019..2257285 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AUO99_RS12290 2252588..2254321 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
AUO99_RS12295 2254327..2255037 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AUO99_RS12300 2255062..2255958 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AUO99_RS12305 2256070..2256591 + 522 WP_001055874.1 flavodoxin FldB -
AUO99_RS12310 2256631..2257038 - 408 WP_000244765.1 protein YgfX Toxin
AUO99_RS12315 2257019..2257285 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
AUO99_RS12320 2257528..2258508 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
AUO99_RS12325 2258585..2259244 - 660 WP_000250275.1 hemolysin III family protein -
AUO99_RS12330 2259408..2259719 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AUO99_RS12335 2259764..2261197 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T59094 WP_000244765.1 NZ_CP013658:c2257038-2256631 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T59094 NZ_CP013658:c2257038-2256631 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT59094 WP_000354050.1 NZ_CP013658:c2257285-2257019 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT59094 NZ_CP013658:c2257285-2257019 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References