59074

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 47579..48104 Replicon plasmid unnamed
Accession NZ_CP013657
Organism Escherichia coli strain uk_P46212

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AUO99_RS00290 Protein ID WP_001159868.1
Coordinates 47799..48104 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AUO99_RS00285 Protein ID WP_000813634.1
Coordinates 47579..47797 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AUO99_RS00260 42858..44471 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
AUO99_RS00265 44502..44852 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
AUO99_RS00270 44849..45274 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
AUO99_RS00275 45336..46487 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
AUO99_RS00280 46521..47033 - 513 WP_000151784.1 hypothetical protein -
AUO99_RS00285 47579..47797 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AUO99_RS00290 47799..48104 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AUO99_RS00295 48105..48911 + 807 WP_000016982.1 site-specific integrase -
AUO99_RS00300 49685..50440 + 756 WP_000852146.1 replication initiation protein RepE -
AUO99_RS00305 51028..52194 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1B / tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1 - 1..143748 143748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T59074 WP_001159868.1 NZ_CP013657:47799-48104 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T59074 NZ_CP013657:47799-48104 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT59074 WP_000813634.1 NZ_CP013657:47579-47797 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT59074 NZ_CP013657:47579-47797 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References