Detailed information of TA system TA059071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 28581..29140
• Replicon: plasmid unnamed
• Accession: NZ_CP013657
• Organism: Escherichia coli strain uk_P46212

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: AUO99_RS00185
• Protein ID: WP_000421272.1
• Coordinates: 28865..29140 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: AUO99_RS00180
• Protein ID: WP_001387467.1
• Coordinates: 28581..28865 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO99_RS00150	24044..24484	-	441	Protein_27	zinc-binding dehydrogenase	-
AUO99_RS00155	24458..24652	+	195	WP_000796505.1	hypothetical protein	-
AUO99_RS00160	24885..25775	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
AUO99_RS00165	25800..26180	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
AUO99_RS00170	26213..27178	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
AUO99_RS00175	27224..27976	-	753	WP_000562172.1	IclR family transcriptional regulator	-
AUO99_RS00180	28581..28865	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
AUO99_RS00185	28865..29140	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AUO99_RS00190	29246..29539	+	294	WP_001105060.1	hypothetical protein	-
AUO99_RS28990	29574..29702	-	129	Protein_36	IS481 family transposase	-
AUO99_RS00195	29735..30903	-	1169	Protein_37	hypothetical protein	-
AUO99_RS00200	31396..31703	+	308	Protein_38	type II toxin-antitoxin system ParD family antitoxin	-
AUO99_RS00210	31992..32689	+	698	Protein_39	IS1 family transposase	-
AUO99_RS00215	32943..33965	-	1023	WP_000361402.1	helicase UvrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1	-	1..143748	143748
	-	flank	IS/Tn	-	-	32186..32689	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T59071 WP_000421272.1 NZ_CP013657:28865-29140 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T59071 NZ_CP013657:28865-29140 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT59071 WP_001387467.1 NZ_CP013657:28581-28865 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT59071 NZ_CP013657:28581-28865 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880