Detailed information of TA system TA059068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4699568..4700203
• Replicon: chromosome
• Accession: NZ_CP013653
• Organism: Paenibacillus sp. 32O-W

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IJ21_RS21210
• Protein ID: WP_062495330.1
• Coordinates: 4699568..4699918 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0U2UUQ0
• Locus tag: IJ21_RS21215
• Protein ID: WP_062495331.1
• Coordinates: 4699922..4700203 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IJ21_RS21190	4694798..4696195	-	1398	WP_062495322.1	Flp pilus assembly complex ATPase component TadA	-
IJ21_RS21195	4696192..4697031	-	840	WP_062495324.1	hypothetical protein	-
IJ21_RS21200	4697028..4697759	-	732	WP_062495326.1	hypothetical protein	-
IJ21_RS21205	4698108..4699469	+	1362	WP_062495328.1	ATP synthase	-
IJ21_RS21210	4699568..4699918	-	351	WP_062495330.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IJ21_RS21215	4699922..4700203	-	282	WP_062495331.1	ribbon-helix-helix protein, CopG family	Antitoxin
IJ21_RS21220	4700405..4701589	-	1185	WP_062495333.1	alanine racemase	-
IJ21_RS21225	4701721..4702971	-	1251	WP_062495335.1	hypothetical protein	-
IJ21_RS21230	4703192..4704874	-	1683	WP_062495337.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12837.90 Da
• Isoelectric Point: 6.2212

>T59068 WP_062495330.1 NZ_CP013653:c4699918-4699568 [Paenibacillus sp. 32O-W]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEINAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMRKVNESLMISVGLIDF

Nucleotide

• Length: 351 bp

>T59068 NZ_CP013653:c4699918-4699568 [Paenibacillus sp. 32O-W]
TTGATTGTAAAACGCGGTGATGTTTTTTTTGCCGACCTATCGCCCGTGGTGGGATCGGAACAAGGCGGAGTGCGGCCGGT
GCTCGTTATCCAGAACGATATCGGCAACCGTTTCAGCCCGACTGTCATTATAGCCGCTATTACGGCTCAAATCCAAAAAG
CGAAACTGCCGACCCATGTCGAAATCAATGCCGCCGCGCACGGATTTGACAGAGACTCGGTCATTTTGTTGGAGCAGATC
CGCACGATCGATAAGCAGCGTTTAACGGACAAAATTACCCATTTGGACGACGACATGATGCGGAAAGTGAACGAATCCCT
TATGATCAGTGTCGGACTGATCGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10689.28 Da
• Isoelectric Point: 6.4721

>AT59068 WP_062495331.1 NZ_CP013653:c4700203-4699922 [Paenibacillus sp. 32O-W]
VANVQNTKRIMISLPDQLLEEVDGIVAKENGNRSEFIRQAMKLYIMERRKRQIRESMQRGYLEMAIINLNMASEAFHAEE
DADHTLGRLVSGV

Nucleotide

• Length: 282 bp

>AT59068 NZ_CP013653:c4700203-4699922 [Paenibacillus sp. 32O-W]
GTGGCCAACGTACAGAATACGAAACGGATTATGATCAGCCTGCCAGACCAGCTGTTAGAGGAAGTTGACGGAATTGTCGC
CAAGGAGAACGGAAATCGCAGCGAATTTATCCGTCAGGCTATGAAGCTGTATATTATGGAGCGCAGAAAGCGGCAAATCC
GGGAATCGATGCAGCGCGGGTACCTCGAGATGGCGATCATCAATCTGAACATGGCCTCGGAAGCTTTTCATGCGGAGGAA
GATGCCGACCATACACTCGGCCGTCTCGTAAGCGGGGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0U2UUQ0