Detailed information of TA system TA059067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 3798037..3798678
• Replicon: chromosome
• Accession: NZ_CP013652
• Organism: Paenibacillus naphthalenovorans strain 32O-Y

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0P9DXL9
• Locus tag: IJ22_RS18615
• Protein ID: WP_010494553.1
• Coordinates: 3798037..3798387 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IJ22_RS18620
• Protein ID: WP_171005911.1
• Coordinates: 3798391..3798678 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IJ22_RS26380	3793257..3793403	+	147	WP_160327392.1	hypothetical protein	-
IJ22_RS18600	3794424..3794897	-	474	WP_062409891.1	SprT family protein	-
IJ22_RS18605	3795047..3795409	+	363	WP_062409892.1	hypothetical protein	-
IJ22_RS26115	3795490..3795630	+	141	WP_137670414.1	cortex morphogenetic protein CmpA	-
IJ22_RS18610	3795738..3797963	-	2226	WP_062409893.1	RNA-binding transcriptional accessory protein	-
IJ22_RS18615	3798037..3798387	-	351	WP_010494553.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IJ22_RS18620	3798391..3798678	-	288	WP_171005911.1	ribbon-helix-helix protein, CopG family	Antitoxin
IJ22_RS18625	3798875..3800062	-	1188	WP_062409894.1	alanine racemase	-
IJ22_RS18630	3800508..3801644	-	1137	WP_054820301.1	outer membrane lipoprotein-sorting protein	-
IJ22_RS18635	3801854..3803386	+	1533	WP_062409895.1	family 43 glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-48)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12904.87 Da
• Isoelectric Point: 4.8943

>T59067 WP_010494553.1 NZ_CP013652:c3798387-3798037 [Paenibacillus naphthalenovorans]
MIVKRGDVFFADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAEAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMRKVDEALQISVGLIEF

Nucleotide

• Length: 351 bp

>T59067 NZ_CP013652:c3798387-3798037 [Paenibacillus naphthalenovorans]
TTGATTGTCAAACGTGGCGATGTCTTTTTCGCGGATCTCTCACCGGTCGTGGGTTCCGAGCAGGGCGGCATTCGTCCTGT
TCTGATCATTCAGAATGATATTGGCAATCGCTTCAGCCCCACGGTGATCGTGGCAGCGATTACAGCACAGATTCAGAAGG
CCAAGCTGCCGACGCACGTGGAGATTGACGCGGAAGCGCACGGTTTTGACAGGGATTCGGTCATTCTGCTAGAACAAATC
CGGACCATTGACAAGCAGCGGTTAACGGATAAAATCACGCACTTGGATGAAGAAACCATGCGCAAGGTCGACGAGGCTTT
GCAAATCAGCGTAGGACTAATTGAGTTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11029.75 Da
• Isoelectric Point: 7.4016

>AT59067 WP_171005911.1 NZ_CP013652:c3798678-3798391 [Paenibacillus naphthalenovorans]
MLVAGAHNTKRIMISLPDYLLKEVDHLVEKENSNRSEFIRQAMKLYLIERKKRHLRDSMQRGYMEMAKINLHIASEAFQA
EEEADGTLDRLVSGV

Nucleotide

• Length: 288 bp

>AT59067 NZ_CP013652:c3798678-3798391 [Paenibacillus naphthalenovorans]
ATGTTGGTGGCGGGTGCGCATAATACGAAGCGAATCATGATCAGCTTGCCGGATTACTTGCTGAAGGAAGTCGACCATCT
GGTGGAAAAGGAAAATTCCAACCGCAGCGAGTTTATCCGTCAGGCAATGAAGCTGTATTTGATTGAGCGAAAAAAGAGGC
ATTTGCGCGATTCCATGCAGCGCGGCTATATGGAAATGGCTAAGATCAACCTACACATAGCCTCGGAAGCATTTCAGGCG
GAGGAAGAAGCGGATGGCACGCTTGACCGTCTTGTAAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: A0A0P9DXL9