Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2531054..2531616 Replicon chromosome
Accession NZ_CP013650
Organism Lacimicrobium alkaliphilum strain YelD216

Toxin (Protein)


Gene name mazF Uniprot ID A0A0U2ZIM6
Locus tag AT746_RS11545 Protein ID WP_062480455.1
Coordinates 2531281..2531616 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0U2JJ47
Locus tag AT746_RS11540 Protein ID WP_062480454.1
Coordinates 2531054..2531284 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT746_RS11500 2526610..2527503 + 894 WP_062480436.1 LysR family transcriptional regulator -
AT746_RS11505 2527786..2528286 + 501 WP_062480438.1 hypothetical protein -
AT746_RS11510 2528279..2528491 + 213 WP_062480440.1 helix-turn-helix transcriptional regulator -
AT746_RS11515 2528708..2529130 + 423 WP_082633246.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
AT746_RS11520 2529278..2529592 - 315 WP_062480442.1 HigA family addiction module antidote protein -
AT746_RS11525 2529607..2529888 - 282 WP_062480443.1 type II toxin-antitoxin system RelE/ParE family toxin -
AT746_RS11530 2530022..2530387 - 366 WP_062480449.1 hypothetical protein -
AT746_RS11540 2531054..2531284 + 231 WP_062480454.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AT746_RS11545 2531281..2531616 + 336 WP_062480455.1 endoribonuclease MazF Toxin
AT746_RS11550 2531884..2534343 - 2460 WP_062480457.1 SIR2 family protein -
AT746_RS11555 2534684..2536552 - 1869 WP_062480459.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

SpoVT-AbrB domain

(7-39)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12111.99 Da        Isoelectric Point: 9.1982

>T59063 WP_062480455.1 NZ_CP013650:2531281-2531616 [Lacimicrobium alkaliphilum]
VSRAPDVGDIVIVDFDPQAGHEQAGKRPALVLSPAQFNKITGFAWLCPVTNQQKGYPFEVKVQGTKRTKGVILTDQLKAL
DWSARNLHKVDKVNAGCLTEVKSLVSTILDI

Download         Length: 336 bp

>T59063 NZ_CP013650:2531281-2531616 [Lacimicrobium alkaliphilum]
GTGAGCCGCGCTCCGGATGTGGGGGATATTGTAATAGTTGATTTTGATCCGCAGGCTGGCCACGAACAAGCGGGAAAGCG
CCCTGCTCTGGTATTAAGCCCGGCACAGTTCAACAAAATCACCGGCTTTGCCTGGCTCTGTCCTGTGACAAATCAGCAAA
AAGGCTATCCATTCGAAGTTAAAGTTCAGGGAACTAAAAGAACTAAAGGGGTTATTCTTACCGATCAGCTGAAAGCGTTG
GATTGGTCGGCAAGAAACCTTCACAAAGTAGATAAAGTCAACGCCGGGTGTTTGACAGAAGTAAAGAGTCTAGTCTCGAC
TATCCTGGATATATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8283.53 Da        Isoelectric Point: 4.9858

>AT59063 WP_062480454.1 NZ_CP013650:2531054-2531284 [Lacimicrobium alkaliphilum]
MTASIAKWGNSAALRLPKNILDALSLNIGDKVNIVQKGGCVVIEPCKPSLDELLAQVTPENRHEEVFNDQTGRELL

Download         Length: 231 bp

>AT59063 NZ_CP013650:2531054-2531284 [Lacimicrobium alkaliphilum]
ATGACCGCATCAATTGCAAAATGGGGTAATAGCGCGGCCCTTCGGCTGCCTAAAAATATTCTTGATGCGCTATCTTTGAA
TATTGGCGACAAGGTGAATATTGTTCAGAAAGGCGGATGTGTTGTGATTGAGCCCTGCAAGCCCTCGCTGGACGAACTGC
TCGCGCAAGTCACGCCTGAGAACCGGCACGAAGAGGTGTTCAACGACCAGACCGGGCGTGAACTCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U2ZIM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0U2JJ47

References