59060

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 431512..432047 Replicon plasmid pRspN324e
Accession NZ_CP013635
Organism Rhizobium sp. N324

Toxin (Protein)


Gene name relE Uniprot ID A0A235AIH9
Locus tag AMK05_RS31900 Protein ID WP_064844553.1
Coordinates 431512..431787 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A235AJT9
Locus tag AMK05_RS31905 Protein ID WP_064844555.1
Coordinates 431787..432047 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK05_RS31880 426620..427546 - 927 WP_064845016.1 sugar ABC transporter permease -
AMK05_RS31885 427643..428863 - 1221 WP_064844546.1 sugar ABC transporter substrate-binding protein -
AMK05_RS31890 428913..430232 - 1320 WP_064844549.1 alpha-L-fucosidase -
AMK05_RS31895 430439..431446 + 1008 WP_064844551.1 LacI family transcriptional regulator -
AMK05_RS31900 431512..431787 - 276 WP_064844553.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMK05_RS31905 431787..432047 - 261 WP_064844555.1 ribbon-helix-helix domain-containing protein Antitoxin
AMK05_RS31910 432248..432976 + 729 WP_064844557.1 FadR family transcriptional regulator -
AMK05_RS31915 433267..434280 + 1014 WP_064844559.1 ABC transporter substrate-binding protein -
AMK05_RS31920 434375..435193 + 819 WP_064844561.1 ABC transporter ATP-binding protein -
AMK05_RS31925 435205..436086 + 882 WP_064844564.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..667630 667630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10746.39 Da        Isoelectric Point: 9.9718

>T59060 WP_064844553.1 NZ_CP013635:c431787-431512 [Rhizobium sp. N324]
MELKWTSKAVSDIGRLYAFLAPVNRQAAARTVQALTAAPLRLLEQPRLGERLEEFDPREVRRILVGHYELRYEIQQSAIY
VLRLWHTREDR

Download         Length: 276 bp

>T59060 NZ_CP013635:c431787-431512 [Rhizobium sp. N324]
ATGGAACTCAAATGGACGAGCAAAGCGGTCTCGGACATTGGCCGCCTCTATGCCTTTCTCGCTCCGGTCAATCGACAGGC
GGCGGCTCGGACTGTGCAGGCCTTGACGGCAGCGCCGCTGCGGCTGCTTGAGCAGCCGCGCCTTGGCGAGCGGCTGGAGG
AGTTCGATCCTCGGGAGGTCCGCCGCATTCTCGTTGGTCACTACGAGCTACGTTATGAAATACAGCAGTCGGCCATCTAT
GTATTGCGGCTCTGGCATACGCGTGAAGACCGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9658.92 Da        Isoelectric Point: 4.8182

>AT59060 WP_064844555.1 NZ_CP013635:c432047-431787 [Rhizobium sp. N324]
METKVLTAHVPLSLAQKVDQLAARLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDEGKVVDHQSVQAWADSLDSDA
PVSLPR

Download         Length: 261 bp

>AT59060 NZ_CP013635:c432047-431787 [Rhizobium sp. N324]
ATGGAAACCAAAGTGCTGACTGCGCATGTTCCCCTGTCCCTTGCGCAAAAGGTCGATCAACTCGCCGCGAGGCTTGAGCG
CTCGCGCGGCTGGATCGTCAAGCAGGCGTTGACGGCATGGATCGATCAGGAGGAGGAGCGTCGCCGTTTGACGCTGGAAG
CGCTGGCTGATGTGGATGAAGGCAAAGTGGTCGATCATCAGTCCGTCCAGGCCTGGGCCGATAGTCTCGACAGTGACGCG
CCGGTTTCGCTGCCTCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A235AIH9

Antitoxin

Source ID Structure
AlphaFold DB A0A235AJT9

References