Detailed information of TA system TA059059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 57232..57902
• Replicon: plasmid pRspN324e
• Accession: NZ_CP013635
• Organism: Rhizobium sp. N324

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A235AHI1
• Locus tag: AMK05_RS30265
• Protein ID: WP_064843896.1
• Coordinates: 57483..57902 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AMK05_RS30260
• Protein ID: WP_064844955.1
• Coordinates: 57232..57486 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK05_RS30240	52488..53381	+	894	WP_064843893.1	carbohydrate ABC transporter permease	-
AMK05_RS30245	53386..55104	+	1719	WP_064844953.1	GH32 C-terminal domain-containing protein	-
AMK05_RS30250	55156..56232	-	1077	WP_064843894.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
AMK05_RS30255	56336..56932	+	597	WP_064843895.1	TetR/AcrR family transcriptional regulator	-
AMK05_RS30260	57232..57486	+	255	WP_064844955.1	hypothetical protein	Antitoxin
AMK05_RS30265	57483..57902	+	420	WP_064843896.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMK05_RS30270	58002..59996	-	1995	WP_064843897.1	glycosyltransferase	-
AMK05_RS30275	59978..61210	-	1233	WP_064844957.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AMK05_RS30280	61630..61830	-	201	WP_064843898.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..667630	667630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15089.46 Da
• Isoelectric Point: 5.8701

>T59059 WP_064843896.1 NZ_CP013635:57483-57902 [Rhizobium sp. N324]
MILLDTNVISEPWKPVPEGAVVAWLDAQTLETLFLSAITIAELRFGIAAMPSGKRQTILRDRLEDEVLPHFAKRILPFDF
AASQFYSELMARARVAGKAISKADGYIAATAAANGFAVATRDTSPFEAAGLKVINPWTP

Nucleotide

• Length: 420 bp

>T59059 NZ_CP013635:57483-57902 [Rhizobium sp. N324]
ATGATCTTGTTGGACACCAACGTGATATCGGAGCCGTGGAAACCGGTTCCTGAGGGAGCGGTGGTCGCTTGGTTGGACGC
CCAAACCCTCGAGACTCTCTTCCTTTCCGCAATAACAATTGCCGAACTTCGTTTCGGGATCGCTGCTATGCCCTCAGGCA
AACGGCAAACTATCCTTCGCGATCGCCTGGAGGATGAAGTGCTTCCGCATTTTGCCAAACGCATCCTGCCTTTCGATTTT
GCCGCCAGTCAATTCTACTCGGAACTGATGGCACGCGCTCGAGTAGCCGGGAAGGCGATCAGCAAGGCGGATGGATATAT
TGCAGCCACCGCAGCAGCGAACGGCTTTGCCGTCGCAACTCGCGACACAAGCCCTTTTGAGGCCGCTGGGTTGAAAGTGA
TTAACCCCTGGACTCCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9273.39 Da
• Isoelectric Point: 6.2260

>AT59059 WP_064844955.1 NZ_CP013635:57232-57486 [Rhizobium sp. N324]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPSERVKLGSLLATIGRDAELSNSDVEALQQNRDKGPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT59059 NZ_CP013635:57232-57486 [Rhizobium sp. N324]
ATGCCGGCGGTCACCATTCGCAATCTCTCTGATGAAACACATCGCGCCCTGCGGGTGCGAGCGGCTCATCATGGCCGAAG
CACAGAGGCCGAAATCCGTGACATTATCGAGGCCGCCGTTCGCCCATCTGAACGCGTGAAGCTCGGCTCTTTACTCGCGA
CCATTGGTCGTGATGCTGAGCTTTCCAATAGCGACGTTGAAGCCCTGCAGCAAAATCGCGATAAGGGGCCTGCTGAACCC
ATGACTTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A235AHI1