59057

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 96056..96726 Replicon plasmid pRspN324a
Accession NZ_CP013631
Organism Rhizobium sp. N324

Toxin (Protein)


Gene name vapC Uniprot ID A0A235AN05
Locus tag AMK05_RS22735 Protein ID WP_064841573.1
Coordinates 96056..96460 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A235AMX6
Locus tag AMK05_RS22740 Protein ID WP_064841574.1
Coordinates 96457..96726 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK05_RS22710 91807..92112 - 306 WP_064841765.1 hypothetical protein -
AMK05_RS22715 92096..93583 - 1488 WP_064841570.1 type II and III secretion system protein family protein -
AMK05_RS22720 93642..94559 - 918 WP_064841571.1 Flp pilus assembly protein CpaB -
AMK05_RS22725 94658..94855 - 198 WP_064841572.1 hypothetical protein -
AMK05_RS22730 95115..95645 + 531 WP_064841766.1 prepilin peptidase -
AMK05_RS22735 96056..96460 - 405 WP_064841573.1 type II toxin-antitoxin system VapC family toxin Toxin
AMK05_RS22740 96457..96726 - 270 WP_064841574.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AMK05_RS22745 96870..97166 - 297 WP_064841767.1 MurR/RpiR family transcriptional regulator -
AMK05_RS22750 97303..98295 - 993 WP_064841575.1 inositol 2-dehydrogenase -
AMK05_RS22755 98338..99141 - 804 WP_064841576.1 TIM barrel protein -
AMK05_RS22760 99219..100013 - 795 WP_064841577.1 sugar ABC transporter ATP-binding protein -
AMK05_RS22765 100016..101137 - 1122 WP_064841578.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..305839 305839


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

SpoVT-AbrB domain

(18-55)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14143.50 Da        Isoelectric Point: 5.1418

>T59057 WP_064841573.1 NZ_CP013631:c96460-96056 [Rhizobium sp. N324]
VTSLYMLDTNIVSELARNPRGVVARRIAEVGAEAICVSIITASELRYGCAKKGSAKLLAQIEAILGSIPVLALDVPADAQ
YGVIRAELETCGKPIGPNDLFIAAHACALGAVLVTANLGEFVRVRALQVENWLD

Download         Length: 405 bp

>T59057 NZ_CP013631:c96460-96056 [Rhizobium sp. N324]
GTGACCAGCCTTTATATGCTGGACACCAATATCGTGTCCGAGCTTGCTCGAAACCCGAGGGGCGTTGTCGCTCGACGCAT
CGCTGAGGTCGGCGCGGAAGCAATCTGCGTCAGCATCATCACGGCATCTGAGTTGCGCTACGGTTGCGCCAAGAAAGGAT
CGGCGAAACTTCTCGCACAGATCGAGGCCATCCTTGGGAGCATCCCCGTGCTGGCACTGGATGTGCCCGCCGACGCCCAG
TATGGCGTCATCCGGGCGGAACTAGAGACCTGTGGCAAACCTATCGGTCCGAATGACCTCTTCATTGCCGCGCATGCATG
CGCCTTGGGAGCAGTTCTTGTGACGGCGAACCTCGGAGAGTTCGTGCGCGTGCGCGCTTTGCAGGTCGAGAACTGGTTGG
ATTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10196.69 Da        Isoelectric Point: 5.6328

>AT59057 WP_064841574.1 NZ_CP013631:c96726-96457 [Rhizobium sp. N324]
MPHLRHNETTPPREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLILEPVRRKNLLEVLAGLQPLGPEDQFPDVDET
LLPAKDIDL

Download         Length: 270 bp

>AT59057 NZ_CP013631:c96726-96457 [Rhizobium sp. N324]
ATGCCCCATCTCCGGCACAATGAAACCACCCCGCCACGGGAGGCTAAGCTTTTCCGAAACAACAAAAGCCAAGCTGTGCG
AATTCCGGCTGACTTTGAATTGCCCGGCGACCGAGTGATGATCCACAGGGATGGTGATCGGCTCATCCTAGAGCCGGTCC
GACGCAAGAACCTCCTAGAAGTACTAGCCGGTTTGCAGCCGCTTGGTCCCGAAGATCAGTTCCCGGATGTTGACGAGACC
TTGCTTCCCGCGAAGGATATCGACTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A235AN05

Antitoxin

Source ID Structure
AlphaFold DB A0A235AMX6

References