59056

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 4287844..4288406 Replicon chromosome
Accession NZ_CP013630
Organism Rhizobium sp. N324

Toxin (Protein)


Gene name brnT Uniprot ID A0A235AQJ8
Locus tag AMK05_RS20645 Protein ID WP_064840925.1
Coordinates 4287844..4288092 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A235ANT2
Locus tag AMK05_RS20650 Protein ID WP_064840926.1
Coordinates 4288089..4288406 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK05_RS20620 4283798..4284931 - 1134 WP_064840922.1 D-alanyl-D-alanine carboxypeptidase -
AMK05_RS20625 4285117..4286280 + 1164 WP_064840923.1 amidohydrolase -
AMK05_RS35305 4286612..4286761 + 150 WP_171899808.1 hypothetical protein -
AMK05_RS20635 4286788..4287258 - 471 WP_171899809.1 hypothetical protein -
AMK05_RS33510 4287215..4287780 - 566 Protein_4092 hypothetical protein -
AMK05_RS20645 4287844..4288092 + 249 WP_064840925.1 BrnT family toxin Toxin
AMK05_RS20650 4288089..4288406 + 318 WP_064840926.1 BrnA antitoxin family protein Antitoxin
AMK05_RS20655 4288527..4289003 - 477 WP_064840927.1 DUF2937 family protein -
AMK05_RS20660 4289169..4289432 + 264 WP_064840928.1 hypothetical protein -
AMK05_RS20665 4289726..4290016 - 291 WP_064840929.1 BON domain-containing protein -
AMK05_RS20670 4290222..4290872 - 651 WP_064841471.1 hypothetical protein -
AMK05_RS34695 4290981..4291160 - 180 WP_065092533.1 hypothetical protein -
AMK05_RS20675 4291232..4292422 + 1191 WP_064841472.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-103)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9147.63 Da        Isoelectric Point: 7.0136

>T59056 WP_064840925.1 NZ_CP013630:4287844-4288092 [Rhizobium sp. N324]
MEIVWDEPKRIANIEKHGLDFADLTFEFFLSSVVVPAKDGRSKAIGRLADGTIVAIFVNLGSEALSIISMRPARKDERSM
IR

Download         Length: 249 bp

>T59056 NZ_CP013630:4287844-4288092 [Rhizobium sp. N324]
ATGGAGATCGTATGGGACGAACCGAAGCGGATTGCCAATATCGAGAAACATGGCCTCGATTTCGCCGATCTGACTTTCGA
GTTCTTCCTGTCTTCCGTTGTCGTTCCCGCCAAAGATGGGCGATCCAAGGCCATAGGCCGCCTCGCTGACGGAACGATCG
TCGCAATCTTCGTAAATCTGGGCTCTGAGGCGCTTTCGATCATTTCAATGCGCCCCGCCCGCAAGGACGAAAGGAGCATG
ATCCGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11832.60 Da        Isoelectric Point: 10.5655

>AT59056 WP_064840926.1 NZ_CP013630:4288089-4288406 [Rhizobium sp. N324]
MIIKARKTTQFQPGRGYSKEDWDAVSDNPPLSKTEMANAKPFKEAFPEVAEKMERAIAARGRPKLDNPKQPLNIRLDADI
IQFYKATGKGWQSRMNDALRKAAGL

Download         Length: 318 bp

>AT59056 NZ_CP013630:4288089-4288406 [Rhizobium sp. N324]
ATGATTATCAAAGCCCGGAAAACGACGCAGTTCCAGCCTGGCCGCGGCTACTCCAAGGAGGATTGGGATGCCGTCTCCGA
CAATCCCCCTCTGTCCAAGACAGAGATGGCAAACGCCAAGCCCTTCAAGGAGGCATTTCCTGAGGTTGCCGAAAAGATGG
AAAGGGCCATTGCAGCCCGCGGTCGACCCAAGCTGGACAACCCGAAGCAACCCCTGAACATTCGTCTCGATGCCGATATC
ATCCAGTTTTACAAGGCCACTGGCAAAGGTTGGCAGTCAAGGATGAACGATGCATTGCGAAAGGCGGCTGGCTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A235AQJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A235ANT2

References