Detailed information of TA system TA059053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1141072..1141549
• Replicon: chromosome
• Accession: NZ_CP013630
• Organism: Rhizobium sp. N324

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: AMK05_RS05440
• Protein ID: WP_064837225.1
• Coordinates: 1141280..1141549 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A235ASV3
• Locus tag: AMK05_RS05435
• Protein ID: WP_064837223.1
• Coordinates: 1141072..1141296 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK05_RS05415	1136266..1137270	+	1005	WP_064837215.1	LacI family transcriptional regulator	-
AMK05_RS05420	1137271..1138758	+	1488	WP_064837217.1	sugar ABC transporter ATP-binding protein	-
AMK05_RS05425	1138772..1139707	+	936	WP_064837219.1	ABC transporter permease	-
AMK05_RS05430	1139734..1140735	+	1002	WP_064837221.1	substrate-binding domain-containing protein	-
AMK05_RS05435	1141072..1141296	+	225	WP_064837223.1	TraY domain-containing protein	Antitoxin
AMK05_RS05440	1141280..1141549	+	270	WP_064837225.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMK05_RS05445	1141699..1142472	+	774	WP_064837228.1	DsbA family protein	-
AMK05_RS05450	1142728..1146189	+	3462	WP_064837230.1	chromosome segregation protein SMC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10387.91 Da
• Isoelectric Point: 8.5165

>T59053 WP_064837225.1 NZ_CP013630:1141280-1141549 [Rhizobium sp. N324]
MAWRIEFHRAAERELEKLGHEAACRILRFLNDRVARLDDPRSIGEALKGSELGDFWRYRVGDYRVIASIDDGAVRILIVR
IGNRCDVYR

Nucleotide

• Length: 270 bp

>T59053 NZ_CP013630:1141280-1141549 [Rhizobium sp. N324]
ATGGCGTGGAGAATTGAGTTTCACCGGGCAGCCGAGCGTGAGCTTGAGAAGCTCGGACATGAGGCGGCTTGCCGAATCCT
CCGATTTCTCAATGACCGCGTTGCAAGGCTGGACGATCCACGGTCCATCGGAGAGGCGCTTAAAGGGTCTGAACTCGGTG
ATTTCTGGAGGTACCGCGTCGGGGACTATCGAGTGATCGCAAGCATCGACGATGGGGCGGTGCGAATCCTCATCGTGCGT
ATCGGCAACCGCTGCGATGTCTATCGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8607.77 Da
• Isoelectric Point: 4.8894

>AT59053 WP_064837223.1 NZ_CP013630:1141072-1141296 [Rhizobium sp. N324]
MLALRLPPEIEARLDELARRTGRSKSFYARQAILEHLDDLEDIYLAEKRLEELRRGESDTVSLAELMTRHGVEN

Nucleotide

• Length: 225 bp

>AT59053 NZ_CP013630:1141072-1141296 [Rhizobium sp. N324]
ATGCTGGCCTTGCGGTTGCCACCCGAAATCGAAGCACGACTCGATGAGCTTGCCAGGCGGACCGGCCGCAGCAAGAGCTT
CTATGCCCGTCAGGCCATCCTCGAACATCTCGATGATCTCGAGGATATCTATCTCGCCGAAAAACGCCTCGAAGAGCTTC
GGCGAGGGGAGAGTGATACCGTTTCGCTGGCCGAGTTGATGACGCGTCATGGCGTGGAGAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A235ASV3