Detailed information of TA system TA059052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 165466..166055
• Replicon: chromosome
• Accession: NZ_CP013630
• Organism: Rhizobium sp. N324

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AMK05_RS00750
• Protein ID: WP_064835662.1
• Coordinates: 165466..165663 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A235AQ61
• Locus tag: AMK05_RS00755
• Protein ID: WP_064835664.1
• Coordinates: 165660..166055 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK05_RS00720	160804..161277	-	474	WP_064835650.1	hypothetical protein	-
AMK05_RS00725	161391..162026	+	636	WP_064835652.1	trimeric intracellular cation channel family protein	-
AMK05_RS00730	162039..162458	-	420	WP_064835654.1	transcriptional repressor	-
AMK05_RS00735	162758..163273	+	516	WP_064835656.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
AMK05_RS00740	163317..164549	+	1233	WP_064835658.1	beta-ketoacyl-ACP synthase I	-
AMK05_RS00745	164554..165360	+	807	WP_064835660.1	enoyl-ACP reductase FabI	-
AMK05_RS00750	165466..165663	+	198	WP_064835662.1	type II toxin-antitoxin system HicA family toxin	Toxin
AMK05_RS00755	165660..166055	+	396	WP_064835664.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AMK05_RS00760	166059..167075	-	1017	WP_064835666.1	class I SAM-dependent methyltransferase	-
AMK05_RS00765	167208..169346	-	2139	WP_064835668.1	polyribonucleotide nucleotidyltransferase	-
AMK05_RS00770	169703..169972	-	270	WP_025417456.1	30S ribosomal protein S15	-
AMK05_RS00775	170125..170976	-	852	WP_064835670.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7185.31 Da
• Isoelectric Point: 11.2092

>T59052 WP_064835662.1 NZ_CP013630:165466-165663 [Rhizobium sp. N324]
VERNSQKIIARLKREGFEVVSIKGSHHKLRRGNETIIVPHPKKDLPTGTALAIAKQAGWSVSDKS

Nucleotide

• Length: 198 bp

>T59052 NZ_CP013630:165466-165663 [Rhizobium sp. N324]
GTGGAGCGAAACAGCCAGAAGATTATTGCCAGGCTGAAGCGGGAAGGCTTCGAAGTCGTTTCGATCAAAGGCTCTCATCA
TAAGCTTCGAAGGGGCAATGAGACCATCATCGTTCCGCACCCGAAAAAGGATCTGCCGACCGGCACCGCGCTTGCAATTG
CGAAGCAGGCCGGATGGAGTGTGAGTGACAAATCATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14397.17 Da
• Isoelectric Point: 4.3257

>AT59052 WP_064835664.1 NZ_CP013630:165660-166055 [Rhizobium sp. N324]
MKYFIALVHKDSDSAFGISFPDLPAVFSAADEEEDLTANAIEALRLWAEDETLPLPSSYDEIGSRQDIRAQLAEGAFLTR
VPFIEDTTRTVRANVTFDKGMLEAIDKAAKERGLTRSAFLASAARKEIEAA

Nucleotide

• Length: 396 bp

>AT59052 NZ_CP013630:165660-166055 [Rhizobium sp. N324]
ATGAAATATTTCATTGCTCTCGTGCATAAGGACAGCGACAGCGCTTTCGGGATCAGCTTTCCGGATCTGCCGGCGGTATT
CTCCGCTGCCGATGAGGAAGAAGACCTTACTGCGAACGCCATAGAAGCCCTTCGCCTTTGGGCGGAAGACGAAACTCTGC
CGCTGCCTTCCTCATATGACGAGATCGGCTCACGGCAGGATATCCGCGCGCAGCTGGCCGAGGGTGCTTTTCTGACGCGC
GTTCCTTTTATCGAAGACACAACACGCACCGTTCGAGCCAATGTGACTTTCGACAAAGGTATGCTTGAGGCAATCGACAA
GGCTGCGAAAGAGCGCGGCCTCACCCGTTCGGCTTTCCTTGCGAGTGCGGCCCGCAAGGAAATCGAGGCTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A235AQ61